Midkine is highly expressed in neuroblastoma tissues
Midkine is highly expressed in neuroblastoma tissues
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DOI：
10.1007/s00383-008-2263-0
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发表时间：
2008-12-01
影响因子：
1.8
通讯作者：
Till, Holger
中科院分区：
文献类型：
--
作者：
Fiegel, Henning C.;Kaifi, Jussuf T.;Till, Holger
目的神经母细胞瘤（NBL）是一种来源于神经嵴细胞的肿瘤，是儿童最常见的实体瘤。中期因子（MK）是一种多效激素类似物，其经常在神经元和上皮肿瘤中表达，并且是不良临床结果的标志物。本研究的目的是评估MK在NBL中的表达，并探讨其与临床outcome.Methods的相关性五十六例NBL标本进行了MK的组织芯片免疫组化染色（IHC）。新鲜冷冻的肿瘤组织用于RNA分离，并进行MK-mRNA表达的RT-PCR分析。生存数据，危险因素和疾病阶段与免疫组化和RT-PCR分析评估MK状态相关。结果MK-mRNA在大多数肿瘤组织中表达（75%），而MK蛋白仅在46%的NBL中可被检测到。MK状态与生存率、危险因素和疾病分期无相关性。结论大多数NBL表达MK mRNA，但并非所有MK mRNA阳性的肿瘤也显示MK IHC阳性。MK-mRNA的高表达可能为基于腺病毒的基因治疗NBL的新方法提供有希望的靶点。
Purpose Neuroblastoma (NBL) is a tumor from neural crest cells, and is the most frequent solid tumor in children. Midkine (MK) is a pleiotropin analogon, which is frequently expressed in neuronal and epithelial tumors and is a marker for a poor clinical outcome. The aims of this study were to assess MK expression in NBL and investigate the correlation with clinical outcome.Methods Fifty-six specimens of NBL were stained for MK on a tissue microarray by immunohistochemistry (IHC). Fresh frozen tumor tissues were used for RNA isolation, and RT-PCR analysis for MK-mRNA expression was performed. Survival data, risk factors and disease stages were correlated with MK status assessed by IHC and RT-PCR analysis.Results MK-mRNA expression was found in the majority of the tumor tissues (75%), whereas MK protein could be detected only in 46% of the NBL by IHC. No correlation of MK status with survival, risk factors or disease stage was observed.Conclusion A majority of NBL express MK-mRNA, whereas not all MK mRNA positive tumors showed also a positive MK IHC staining. The high expression of MK-mRNA expression might present a promising target for new adenovirus-based gene therapeutic approaches for the treatment of NBL.