Gastrointestinal colonisation of BALB/cA mice by Helicobacter pylori monitored by heparin magnetic separation
Gastrointestinal colonisation of BALB/cA mice by Helicobacter pylori monitored by heparin magnetic separation
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DOI：
10.1016/0928-8244(96)00006-5
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发表时间：
1996-04-01
影响因子：
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通讯作者：
Wadstrom, T
中科院分区：
文献类型：
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作者：
Aleljung, P;Nilsson, HO;Wadstrom, T
免疫活性和免疫缺陷的 BALB/cA 小鼠口服饲喂 10(8) 个集落形成单位的 2 日龄螺旋或球状（12 日龄）幽门螺杆菌菌株 NCTC 11637。免疫活性的 BALB/cA 小鼠也口服饲喂，数量逐渐减少。幽门螺杆菌的螺旋或球状形式。通过肝素磁力分离和随后的酶免疫测定（EIA）来监测感染后34天的胃肠道定植过程，以检测幽门螺杆菌细胞。两种类型的小鼠均被幽门螺杆菌定植。与螺旋形式相比，球状形式的幽门螺杆菌在小鼠体内具有更高的 EIA 吸光度值和更有效的定植。饲喂球状幽门螺杆菌的免疫活性 BALB/cA 小鼠在胃组织病理学样本中表现出急性炎症过程。总之，幽门螺杆菌可以感染免疫功能正常和免疫缺陷的BALB/cA小鼠，并且培养12天后获得的球状体（活的但不可培养的）可以感染BALB/cA小鼠。
Immunocompetent and immunodeficient BALB/cA mice were fed orally with 10(8) colony forming units of 2-day-old spiral or coccoid (12 days old) Helicobacter pylori strain NCTC 11637. Immunocompetent BALB/cA mice were also fed orally with decreasing numbers. of spiral or coccoid forms of H. pylori. The gastrointestinal colonisation process was monitored for 34 days post-infection by heparin magnetic separation and subsequent enzyme immunoassay (EIA) for the detection of the H. pylori cells. Both mice types were colonised with H. pylori. The coccoid form of H. pylori gave higher EIA absorbance values and more efficient colonisation in the mice than the spiral form. Immunocompetent BALB/cA mice fed with the coccoid form of H. pylori exhibited an acute inflammation process in histopathological samples from the stomachs. In conclusion, H. pylori can infect both immunocompetent as well as immunodeficient BALB/cA mice and coccoids (viable but non-culturable) obtained after 12 days of culturing can infect BALB/cA mice.