Brassinosteroids control AtEXPA5 gene expression in Arabidopsis thaliana
Brassinosteroids control AtEXPA5 gene expression in Arabidopsis thaliana
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DOI：
10.1016/j.phytochem.2009.11.003
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发表时间：
2010-03-01
期刊：
影响因子：
3.8
通讯作者：
Kim, Seong-Ki
中科院分区：
文献类型：
--
作者：
Park, Chan Ho;Kim, Tae-Wuk;Kim, Seong-Ki
为了阐明扩展蛋白A5（AtEXPA 5）在拟南芥生长发育中的时空作用，观察了缺失突变体expA 5-I的表型变化。与野生型植物相比，突变体具有较小的莲座叶。AtEXPA 5主要表达于A.外源油菜素内酯能增强拟南芥中AtEXPA 5的表达，尤其是在花序、茎和花中。AtEXPA 5的表达在油菜素类固醇缺陷突变体（det 2）和信号突变体（bn 1 -301）中减少，而在bzr 1 -1D中增加，bzr 1 - 1D是油菜素类固醇转录因子A双突变体的显性突变体，bzr 1 - 1DXexpA 5 -1，与bzr 1 -1D突变体相比显示出生长减少。双突变体中bzr 1 -1D的油菜唑抗性受损这些发现表明AtEXPA 5是一个生长调节基因，其表达受拟南芥中BZR 1下游的油菜素类固醇信号传导控制（C）2009 Elsevier Ltd版权所有
To elucidate the spatial and temporal roles of EXPANSIN A5 (AtEXPA5) in growth and development of Arabidopsis thaliana, phenotypic alterations in loss-of-function mutants were observed Seedlings of the null mutant, expA5-I, had shorter roots and hypocotyls than those of wild-type plants under both light and dark conditions. Compared to wild-type plants, the mutants had smaller rosette leaves. AtEXPA5 was dominantly expressed in aerial parts of A. thaliana, especially in the inflorescence stems and flowers Expression of AtEXPA5 was enhanced by exogenously applied brassinosteroids. AtEXPA5 expression was reduced in a brassinosteroid-deficient mutant (det2) and a signaling mutant (bn1-301), while it was increased in bzr1-1D, a dominant mutant of a brassinosteroid transcription factor A double mutant, bzr1-1DXexpA5-1, showed reduced growth compared to the bzr1-1D mutant In addition. the brassinazole resistance of bzr1-1D was impaired in the double mutant These findings indicate that AtEXPA5 is a growth-regulating gene whose expression is controlled by brassinosteroid signaling downstream of BZR1 in A thaliana (C) 2009 Elsevier Ltd All rights reserved