In vivo tracing of canonical Wnt signaling in Xenopus tadpoles by means of an inducible transgenic reporter tool
In vivo tracing of canonical Wnt signaling in Xenopus tadpoles by means of an inducible transgenic reporter tool
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DOI：
10.1016/j.febslet.2005.11.084
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发表时间：
2006-01-23
期刊：
影响因子：
3.5
通讯作者：
Vleminckx, K
中科院分区：
文献类型：
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作者：
Denayer, T;Van Roy, F;Vleminckx, K
经典的Wnt途径在胚胎发生和成体生活中反复使用。为了跟踪活生物体中Wnt信号传导的细胞输出，我们设计了一个可诱导的Wnt响应系统，能够通过eGFP表达动态和特异性地报告Wnt通路活性。与以前的体内报告相比，我们的系统基本上避免了连续信号的干扰，保持休眠，直到添加激素，这使得它成为一个有价值的工具，映射典型的Wnt信号在胚胎后阶段。对转基因非洲爪蟾胚胎进行分析，揭示在蝌蚪阶段特定组织和器官中具有高Wnt途径活性的细胞群体。(c)2005年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
The canonical Wnt pathway is recurrently used during embryogenesis and adult life. To track the cellular output of Wnt signaling in a living organism, we designed a hormone-inducible Wnt responsive system, capable to dynamically and specifically report Wnt pathway activities through eGFP expression. In contrast to previous in vivo reporters, our system essentially avoids interference of consecutive signals by remaining dormant until addition of hormone, which makes it a valuable tool to map canonical Wnt signaling in post-embryonic stages. Transgenic Xenopus laevis embryos were analyzed revealing at tadpole stage in specific tissues and organs cell populations with high Wnt pathway activity. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.