Hepatitis B virus genotypes and HBsAg subtypes in refugees and injection drug users in the United States determined by LiPA and monoclonal EIA
Hepatitis B virus genotypes and HBsAg subtypes in refugees and injection drug users in the United States determined by LiPA and monoclonal EIA
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LiPA 和单克隆 EIA 测定美国难民和注射吸毒者中的乙型肝炎病毒基因型和 HBsAg 亚型
DOI：
10.1002/jmv.1051
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发表时间：
2001
影响因子：
12.7
通讯作者：
L. Stuyver
中科院分区：
文献类型：
--
作者：
P. Swenson;C. van Geyt;E. Alexander;H. Hagan;Jayne M. Freitag‐Koontz;Shari Wilson;H. Norder;L. Magnius;L. Stuyver
对196名HBsAg阳性患者的血清进行了B型肝炎病毒（HBV）基因分型和B型肝炎表面抗原（HBsAg）亚型分型，其中包括151名进入美国的难民和45名西雅图的注射吸毒者。使用一组单克隆抗体通过酶免疫测定法（EIA）进行HBsAg亚型分型，通过聚合酶链反应（PCR）确定HBV基因型，然后使用基因型特异性探针通过反相杂交线性探针试验（利帕）检测扩增的HBV DNA。196份血清中155份（79%）用PCR法检出HBV DNA，155份均用利帕法进行基因分型。来自东南亚的样本主要为基因型B/亚型ayw 1和基因型C/adr;来自前苏联和东欧的样本主要为基因型D/ayw 2和基因型D/ayw 3;来自东非的样本主要为基因型A/adw 2和基因型D/ayw 2;来自注射吸毒者的样本主要为基因型D/ayw 3和基因型A/adw 2。由于氨基酸残基118处的瓦尔/Ala而不是Thr和残基125处的Thr而不是Met，ayw 3的一些菌株在亚型分型试验中产生非典型的单克隆抗体反应性模式。ayw 2的菌株也给出了非典型的单克隆抗体反应模式，这是由于在氨基酸残基123处的Ala而不是Thr。利帕基因分型和单克隆EIA分型可为流行病学研究提供有用的信息。医学病毒学杂志64：305-311，2001.© 2001 Wiley利斯公司
Hepatitis B virus (HBV) genotyping and hepatitis B surface antigen (HBsAg) subtyping were carried out on sera from 196 HBsAg‐positive patients, including 151 refugees entering the United States and 45 injection drug users in Seattle. HBsAg subtyping was performed by enzyme immunoassay (EIA) using a panel of monoclonal antibodies and the HBV genotype was determined by polymerase chain reaction (PCR) followed by detection of amplified HBV DNA by a reverse‐phase hybridization line probe assay (LiPA) using genotype‐specific probes. HBV DNA was detected by PCR in 155 (79%) of the 196 sera and all 155 were genotyped by LiPA. Samples from Southeast Asia were predominantly genotype B/subtype ayw1 and genotype C/adr; samples from the former Soviet Union and eastern Europe were mostly genotype D/ayw2 and genotype D/ayw3; samples from east Africa were mainly genotype A/adw2 and genotype D/ayw2; and samples from injection drug users were mostly genotype D/ayw3 and genotype A/adw2. Some strains of ayw3 gave atypical monoclonal antibody reactivity patterns in the subtyping assay due to a Val/Ala instead of a Thr at amino acid residue 118 and a Thr instead of a Met at residue 125. A strain of ayw2 also gave an atypical monoclonal antibody reactivity pattern due to an Ala instead of a Thr at amino acid residue 123. LiPA genotyping and monoclonal EIA subtyping can provide useful information for epidemiological studies. J. Med. Virol. 64:305–311, 2001. © 2001 Wiley‐Liss, Inc.