Rickettsia peacockii sp nov, a new species infecting wood ticks, Dermacentor andersoni, in Western Montana
Rickettsia peacockii sp nov, a new species infecting wood ticks, Dermacentor andersoni, in Western Montana
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DOI：
10.1099/00207713-47-2-446
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发表时间：
1997-04-01
期刊：
影响因子：
--
通讯作者：
Schwan, TG
中科院分区：
文献类型：
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作者：
Niebylski, ML;Schrumpf, ME;Schwan, TG
孔雀立克次体是斑疹热群立克次体的一个新种，是从蒙大拿州比特鲁谷东侧蓝宝石山脉采集的落基山木蜱（Dermacentor andersoni）中鉴定出来的，通过 PCR 检测，使用源自真细菌 16S 核糖体 DNA (rDNA) 的引物组，扩增了自然感染蜱组织中孔雀立克次体 Skalkaho(T)（T = 型菌株）的 DNA，立克次氏体柠檬酸合成酶和 190-kDa 表面抗原 (rOmpA) 基因，部分 16S rDNA 和 rOmpA 基因序列与斑疹热病原立克次氏体 R 的序列的相似性水平分别为 99.7% 和 93.2%，通过使用 Gimenez 染色、荧光抗体测试、PCR 测定和限制性片段长度多态性分析，115 名女性中的 76 名女性1992年至1995年间采集的蜱虫（最低现场感染率66.1%）被发现感染。该生物体通过跨卵和跨卵巢传播（垂直传播率最低，73.3%），感染局限于卵巢组织。培养孔雀孔雀菌的尝试未成功。
Rickettsia peacockii, a new species of spotted fever group rickettsiae, was identified from Rocky Mountain wood ticks (Dermacentor andersoni) collected in the Sapphire Mountain Range on the eastern side of Bitterroot Valley, Montana, DNA from R, peacockii Skalkaho(T) (T = type strain) in naturally infected tick tissue was amplified by a PCR assay with primer sets derived from eubacterial 16S ribosomal DNA (rDNA), rickettsial citrate synthase, and 190-kDa surface antigen (rOmpA) genes, Partial 16S rDNA and rOmpA gene sequences exhibited levels of similarity of 99.7 and 93.2%, respectively, with the sequences of the spotted fever agent Rickettsia rickettsii R, By using Gimenez staining, fluorescent antibody tests, a PCR assay, and a restriction fragment length polymorphism analysis, 76 of 115 female ticks (minimal field infection rate, 66.1%) collected between 1992 and 1995 were found to be infected. The organism is passed transstadially and transovarially (minimal vertical transmission rate, 73.3%), and infections are localized in ovarial tissues, Attempts to cultivate R. peacockii were unsuccessful.