Expression of ErbB receptors and mucins in the airways of long term current smokers
Expression of ErbB receptors and mucins in the airways of long term current smokers
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DOI：
10.1136/thx.2004.028043
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发表时间：
2004-12-01
期刊：
影响因子：
10
通讯作者：
Wilson, SJ
中科院分区：
文献类型：
--
作者：
O'Donnell, RA;Richter, A;Wilson, SJ
背景：已知慢性吸烟者中存在气道上皮杯状细胞增生。虽然表皮生长因子受体参与了这一过程，但在当前吸烟者中，ErbB受体的表达和上皮的粘液分泌表型都没有被表征。方法：取非吸烟者（n = 10）和当前吸烟者（n = 51）的支气管活检，伴或不伴慢性阻塞性肺疾病（n = 51），采用免疫组织化学方法检测支气管上皮中表皮生长因子受体、ErbB2、ErbB3、ErbB4和粘蛋白亚型（MUC2、MUC5AC和MUC5B）的表达。结果与气道壁和诱导痰中性粒细胞计数相关。结果：除PAS染色外，所有吸烟者与非吸烟者相比，表皮生长因子受体、ErbB3和MUC5AC的表达均显著增加，无论是否存在慢性阻塞性肺疾病。MUC5AC表达与PAS染色和ErbB3表达均显著相关；黏液蛋白或ErbB受体的表达与中性粒细胞计数没有相关性。结论：长期吸烟可诱导体内表皮生长因子受体、ErbB3和MUC5AC表达增强；这些增加与中性粒细胞炎症的存在无关。ErbB受体可能参与上皮细胞对香烟烟雾的反应。
Background: Airway epithelial goblet cell hyperplasia is known to occur in chronic smokers. Although the epidermal growth factor receptor has been implicated in this process, neither ErbB receptor expression nor the mucosecretory phenotype of the epithelium have been characterised in current smokers.Methods: Bronchial biopsies obtained from non-smokers (n = 10) and current smokers, with or without chronic obstructive pulmonary disease ( n = 51), were examined immunohistochemically to measure the expression of epidermal growth factor receptor, ErbB2, ErbB3, ErbB4 and mucin subtypes (MUC2, MUC5AC and MUC5B) in the bronchial epithelium. The results were correlated with neutrophil counts measured in the airway wall and induced sputum.Results: Epidermal growth factor receptor, ErbB3 and MUC5AC expression, in addition to PAS staining, were significantly increased in all smokers compared with non-smokers, irrespective of the presence of chronic obstructive pulmonary disease. MUC5AC expression was significantly associated with both PAS staining and ErbB3 expression; no correlation was observed between either mucin or ErbB receptor expression and neutrophil counts.Conclusions: The results suggest that long term current smoking induces enhanced epidermal growth factor receptor, ErbB3, and MUC5AC expression in vivo; these increases are not associated with the presence of neutrophilic inflammation. ErbB receptors may contribute to epithelial responses to cigarette smoke.