Development of UPLC-Q-TOF-MS Coupled with Cation-exchange Solid-phase Extraction Method for the Determination of Ten Pyrrolizidine Alkaloids in Herbal Medicines
Development of UPLC-Q-TOF-MS Coupled with Cation-exchange Solid-phase Extraction Method for the Determination of Ten Pyrrolizidine Alkaloids in Herbal Medicines
复制标题
UPLC-Q-TOF-MS 联用阳离子交换固相萃取法测定中草药中十种吡咯里西啶生物碱的建立。
DOI：
10.2116/analsci.19p230
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发表时间：
2019-12-01
影响因子：
1.6
通讯作者：
Fu, Qiang
中科院分区：
文献类型：
--
作者：
Luo, Zhimin;Li, Xuan;Fu, Qiang
吡咯里西定生物碱是植物的次生代谢物，对人体有明显的肝毒性。建立了一种快速、简便的超高效液相色谱-四极杆飞行时间质谱仪(UPLC-Q-TOFMS)测定6种中草药中10种吡咯里西丁生物碱的方法。采用强阳离子交换柱实现了高效的固相萃取，并对萃取参数进行了优化。对所建立的分析方法进行了验证，结果表明，该方法具有良好的准确度和精密度。所建立的方法成功地用于千里光、紫草根、紫草根、紫草花、千里光等6种中草药中对苯二甲酸的测定。在所有这些草本植物样品中都发现了多胺。还检出了8种相关商品中草药，其中6种检出了不同含量的PAS。这项工作不仅为中草药中多胺的定性和定量分析提供了一个强大的技术平台，而且获得了这些中草药样品中多胺的相关信息，可以为政府监管部门和非政府组织提供参考。
Pyrrolizidine alkaloids are secondary metabolites of plants and can cause significant hepatotoxicity in humans. In this study, a fast and simple method was developed to determine ten pyrrolizidine alkaloids (PAs) in six types of herbal medicines using ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOFMS). An efficient solid-phase extraction procedure was carried out by using strong cation-exchange cartridges and the parameters were optimized. The established analytical method was validated and the results showed that the method presented satisfactory accuracy and precision. The established method was successfully applied for the determination of PAs in six herbal plants, including Senecionis Scandentis Hebra , Arnebiae Radix , Asteris Radix Et Rhizoma , Farfarae Flos , Senecionis Cannabifolii Herba and Emilia sonchifolia . PAs were found in all of these herbal plant samples. Eight types of related commercial herbal drugs were also detected, six of them were detected with different amounts of PAs. This work not only provided a powerful technical platform for both qualitative and quantitative analysis of PAs in herbal medicines, but also obtained information concerning PAs in these herbal samples, which could provide reference to the government regulatory authorities and non-governmental organizations.