Digitalized Human Organoid for Wireless Phenotyping.
Digitalized Human Organoid for Wireless Phenotyping.
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DOI：
10.1016/j.isci.2018.05.007
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发表时间：
2018-06-29
期刊：
影响因子：
5.8
通讯作者：
Takebe T
中科院分区：
文献类型：
--
作者：
Kimura M;Azuma M;Zhang RR;Thompson W;Mayhew CN;Takebe T
射频识别（RFID）是一种通过无线提供数字信号来跟踪和跟踪多个环境中的单个对象的具有成本效益且耐用的方法;因此，RFID被广泛应用于许多领域。在这里，我们通过生产紧凑的RFID芯片结合类器官（RiO）来将这一概念应用于生物组织。0.4 mm RFID芯片可重复地集成在来自健康和患病供体的10种不同诱导多能干细胞（iPSC）系的自组装类器官内。我们使用数字化的RiO对RiO池进行表型筛选，然后原位检测每个特定供体。我们的原理验证实验表明，可以通过RFID芯片阅读识别严重脂肪变性表型，并且该表型特异于脂肪性肝炎的遗传疾病。鉴于RFID技术的不断进步，本文概述的数字化原理将扩大类器官医学在药物开发、精准医学和移植应用方面的潜力。自组装培养使RFID集成的类器官具有完整的功能显微表型分析与RFID供体识别相结合利用RFID进行脂肪变性筛查的类器官数字化原理验证细胞生物学;干细胞研究;生物工程
Radio frequency identification (RFID) is a cost-effective and durable method to trace and track individual objects in multiple contexts by wirelessly providing digital signals; RFID is thus widely used in many fields. Here, we implement this concept to biological tissues by producing a compact RFID chip-incorporated organoid (RiO). The 0.4 mm RFID chips are reproducibly integrated inside the self-assembling organoids from 10 different induced pluripotent stem cell (iPSC) lines from healthy and diseased donors. We use the digitalized RiO to conduct a phenotypic screen on a pool of RiO, followed by detection of each specific donor in situ. Our proof-of-principle experiments demonstrated that a severely steatotic phenotype could be identified by RFID chip reading and was specific to a genetic disorder of steatohepatitis. Given evolving advancements surrounding RFID technology, the digitalization principle outlined here will expand organoid medicine potential toward drug development, precision medicine, and transplant applications. Generation of radio frequency identification (RFID) tag integrated human organoids Self-assembly culture enables RFID-integrated organoids with intact functions Microscopic phenotypic assay coupled with donor identification by RFID Proof-of-principle of organoid digitalization with RFID for steatosis screening Cell Biology; Stem Cells Research; Bioengineering
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DOI：
10.1109/hicss.2016.426
发表时间：
2016-01
期刊：
Proceedings of the ... Annual Hawaii International Conference on System Sciences. Annual Hawaii International Conference on System Sciences
影响因子：
--
作者：
Chai PR;Rosen RK;Boyer EW
通讯作者：
Boyer EW
影响因子：
--
作者：
Leung AA;Lou JJ;Mareninov S;Silver SS;Routbort MJ;Riben M;Andrechak G;Yong WH
通讯作者：
Yong WH
影响因子：
1.6
作者：
Want, R
通讯作者：
Want, R
影响因子：
64.5
作者：
Sachs, Norman;de Ligt, Joep;Clevers, Hans
通讯作者：
Clevers, Hans
影响因子：
4.6
作者：
Bergeles, Christos;Yang, Guang-Zhong
通讯作者：
Yang, Guang-Zhong