Interleukin-2 inhibits NMDA receptor-mediated currents directly and may differentially affect subtypes.
Interleukin-2 inhibits NMDA receptor-mediated currents directly and may differentially affect subtypes.
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DOI：
10.1016/j.bbrc.2006.10.047
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发表时间：
2006-12
影响因子：
3.1
通讯作者：
Yi Shen;Li-jun Zhu;Shuang Liu;Su-ya Zhou;Jian‐hong Luo
中科院分区：
文献类型：
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作者：
Yi Shen;Li-jun Zhu;Shuang Liu;Su-ya Zhou;Jian‐hong Luo
采用全细胞膜片钳技术，观察了白细胞介素2（IL 2）对培养的大鼠海马神经元和表达重组NMDA受体的人胚肾（HEK）293细胞N-甲基-D-天冬氨酸（NMDA）受体介导电流（INMDA）的影响。结果发现，IL-2（0.01- 1 ng/ml）可立即显著降低培养神经元的INMDA峰值。有趣的是，在HEK 293细胞中诱导的INMDA峰值也被IL-2抑制。我们还发现，IL-2可使含有NR 2A和NR 2B的NMDA受体介导电流（INR 2A和INR 2B）的峰值幅度分别降低54±5%和30± 4%。这些结果提供了新的证据表明，IL-2诱导快速抑制NMDA受体介导的反应的峰电流与可能的NR 1/NR 2A和NR 1/NR 2B亚型的分化，并建议抑制IL-2和NMDA受体之间的直接相互作用介导的。
Using whole-cell patch-clamp recordings, this study investigated the effects of interleukin-2 (IL-2) on N-methyl-d-aspartate (NMDA) receptor-mediated currents (INMDA) in rat cultured hippocampal neurons and human embryonic kidney (HEK) 293 cells expressing recombinant NMDA receptors. We found that IL-2 (0.01–1ng/ml) immediately and significantly decreased peak INMDAin cultured neurons. Interestingly, the peak INMDAinduced in HEK 293 cells was also inhibited by IL-2. We also found that IL-2 differentially decreased the peak amplitudes of NR2A- and NR2B-containing NMDA receptor-mediated currents (INR2Aand INR2B) by 54±5% and 30±4%, respectively. These results provide new evidence that IL-2 induces rapid inhibition of peak currents of NMDA receptor-mediated responses with possible NR1/NR2A and NR1/NR2B subtype-differentiation, and suggest that the inhibition is mediated by direct interaction between IL-2 and NMDA receptors.