Low-intensity ultrasound stimulation enhances chondrogenic differentiation in alginate culture of mesenchymal stem cells
Low-intensity ultrasound stimulation enhances chondrogenic differentiation in alginate culture of mesenchymal stem cells
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DOI：
10.1111/j.1525-1594.2006.00288.x
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发表时间：
2006-09-01
影响因子：
2.4
通讯作者：
Park, So Ra
中科院分区：
文献类型：
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作者：
Lee, Hyun Jung;Choi, Byung Hyune;Park, So Ra
间充质干细胞（MSCs）被认为是软骨修复的潜在自体来源，因为它们可以在三维（3-D）培养条件下通过转化生长因子- β (tgf - β)处理分化为软骨细胞。然而，临床应用仍需要更有效、更通用的MSCs软骨分化方法。最近，低强度超声（LIUS）被证明可以促进体外骨折愈合和诱导MSCs的体外软骨形成。在这项研究中，我们研究了LIUS在三维海藻酸盐培养中对兔间充质干细胞（rMSCs）软骨形成的影响，以及在单层培养中复制后对软骨形成表型维持的影响。LIUS处理rMSCs增加：(i)基质形成；（ii）软骨形成标志物如ii型胶原、聚集蛋白和Sox-9的表达；（iii）与软骨基质完整性相关的金属蛋白酶-2组织抑制剂的表达；（iv）在单层培养中维持软骨细胞表型的能力。值得注意的是，即使没有tgf - β治疗，LIUS的效果也很明显。这些结果表明，在软骨组织工程中，LIUS处理可能是一种有效且经济的体外诱导MSCs成软骨分化的方法。
Mesenchymal stem cells (MSCs) are regarded as a potential autologous source for cartilage repair, because they can differentiate into chondrocytes by transforming growth factor-beta (TGF-beta) treatment under the 3-dimensional (3-D) culture condition. However, more efficient and versatile methods for chondrogenic differentiation of MSCs are still in demand for its clinical application. Recently, low-intensity ultrasound (LIUS) was shown to enhance fracture healing in vitro and induce chondrogenesis of MSCs in vitro. In this study, we investigated the effects of LIUS on the chondrogenesis of rabbit MSCs (rMSCs) in a 3-D alginate culture and on the maintenance of chondrogenic phenotypes after replating them on a monolayer culture. The LIUS treatment of rMSCs increased: (i) the matrix formation; (ii) the expression of chondrogenic markers such as collagen type II, aggrecan, and Sox-9; (iii) the expression of tissue inhibitor of metalloprotease-2 implicated in the integrity of cartilage matrix; and (iv) the capacity to maintain the chondrogenic phenotypes in a monolayer culture. Notably, LIUS effects were clearly shown even without TGF-beta treatment. These results suggest that LIUS treatment could be an efficient and cost-effective method to induce chondrogenic differentiation of MSCs in vitro for cartilage tissue engineering.