Inhibitory effects of wogonin on the invasion of human breast carcinoma cells by downregulating the expression and activity of matrix metalloproteinase-9
Inhibitory effects of wogonin on the invasion of human breast carcinoma cells by downregulating the expression and activity of matrix metalloproteinase-9
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DOI：
10.1016/j.tox.2011.01.018
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发表时间：
2011-04-11
期刊：
影响因子：
4.5
通讯作者：
Guo, Qinglong
中科院分区：
文献类型：
--
作者：
Chen, Pu;Lu, Na;Guo, Qinglong
汉黄芩素是一种从黄芪中提取的天然单萜类化合物，在体内外均显示出具有治疗肿瘤的潜力。然而，汉黄芩素对肿瘤细胞侵袭的影响仍然知之甚少。本研究通过体外实验研究汉黄芩素对人乳腺癌MDA-MB-231细胞的抗侵袭和抗转移作用。汉黄芩素浓度依赖性地抑制细胞的迁移、粘附和侵袭。汉黄芩素能显著抑制内源性和佛波酯诱导的基质金属蛋白酶-9（MMP-9）的表达和活性，其机制可能与抑制蛋白激酶C（PKC）δ转位和细胞外信号调节激酶（ERK）1/2磷酸化有关。提示汉黄芩素可能通过下调MMP-9的表达和活性而抑制肿瘤细胞的侵袭。可能的靶点是PKC δ和ERK 1/2。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Wogonin, a naturally occurring monoflavonoid extracted from Scutellariae radix, has been shown to possess tumor therapeutic potential in vitro and in vivo. However, the effects of wogonin on tumor cells invasion remains poorly understood. In this study, we performed in vitro experiments to investigate the anti-invasive and anti-metastatic activity of wogonin in MDA-MB-231 human breast carcinoma cells. Wogonin caused a concentration-dependent suppression of cell migration, adhesion and invasion. The mechanism revealed that wogonin significantly inhibited the expression and activity of both endogenous and phorbol-12-myristate-13-acetate (PMA)-induced matrix metalloproteinase-9 (MMP-9) potentially associating with the suppression of translocation of protein kinase C (PKC) delta and phosphorylation of extracellular signal-regulated kinase (ERK1/2). These results suggested that wogonin could inhibit the invasion of tumor cells by downregulating the expression and activity of MMP-9. the possible targets may be PKC delta and ERK1/2. (C) 2011 Elsevier Ireland Ltd. All rights reserved.