Co-localization of hypoxia and apoptosis in irradiated and untreated HCT116 human colon carcinoma xenografts.
Co-localization of hypoxia and apoptosis in irradiated and untreated HCT116 human colon carcinoma xenografts.
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经辐射和未经处理的 HCT116 人结肠癌异种移植物中缺氧和细胞凋亡的共定位。
DOI：
10.1007/978-1-4615-4863-8_72
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发表时间：
1998
影响因子：
--
通讯作者：
Evans,SM
中科院分区：
文献类型：
--
作者：
Koch,CJ;Chasan,JE;Jenkins,WT;Chan,CY;Laughlin,KM;Evans,SM
HCT 116人结肠癌异种移植物在裸鼠中生长。对对照和辐照肿瘤的冷冻切片进行染色并分析缺氧和凋亡的分布和程度。通过2-硝基咪唑EF 5的缺氧依赖性代谢物的免疫组织化学染色来测量组织氧分压。使用染色受损DNA的商业试剂盒评估细胞凋亡。尽管凋亡染色不太可能排除其他形式的细胞死亡（坏死、固缩），但发现所有染色均与接近缺氧的区域相关。
HCT116 human colon carcinoma xenografts were grown in nude mice. Frozen sections of control and irradiated tumors were stained and analysed for the distribution and extent of hypoxia and apoptosis. Tissue oxygen partial pressure was measured by immu-nohistochemical staining of hypoxia-dependent metabolites of the 2-nitroimidazole EF5. Apoptosis was assessed using a commercial kit which stains damaged DNA. Although the apoptosis stain was unlikely to exclude other forms of cell death (necrosis, pyknosis) all staining was found to associate with regions of near anoxia.