A serological survey of Rhodococcus equi infection in foals in central Italy:: comparison of two antigens using an ELISA test

A serological survey of Rhodococcus equi infection in foals in central Italy:: comparison of two antigens using an ELISA test
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DOI:
10.1016/s0147-9571(02)00020-6
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发表时间:
2003-01-01
影响因子:
2
通讯作者:
Valente, C
Valente, C
中科院分区:
医学3区
文献类型:
--
作者:
Cuteri, V;Takai, S;Valente, C

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在意大利中部采集602份马驹血样,进行马红球菌感染的血清学调查。用两种不同的抗原进行ELISA试验,所述抗原是用R.马属,ATCC 33071和ATCC 6939。使用抗原ATCC 33071在81份血清样品(13.45%)(OD大于或等于0.3)中获得阳性反应,使用抗原ATCC 6939在73份血清样品(12.12%)中获得阳性反应。虽然发病率不高,但血清学阳性率约为13%。男性和女性之间没有统计学显著差异。使用抗原33071或抗原6939的ELISA试验是检测抗R抗体的快速和可靠的工具。生马驹的马(C)2002爱思唯尔科技有限公司版权所有。
A serological survey of Rhodococcus equi infection was carried out on 602 blood samples collected from foals in central Italy. The assay Was performed with an ELISA test using two different antigens prepared with reference strains of R. equi, ATCC 33071 and ATCC 6939. A positive reaction was obtained on 81 serum samples (13.45%) (OD greater than or equal to 0.3) using antigen ATCC 33071, and on 73 serum samples (12.12%) using antigen ATCC 6939. Although the frequency of the disease was not high, the serological positivity was about 13%. There was no statistically significant difference between males and females. The ELISA test using either Antigen 33071 or Antigen 6939 is a rapid and reliable tool for detecting antibodies against R. equi in foals. (C) 2002 Elsevier Science Ltd. All rights reserved.