Vitamin E improves the free radical defense system potential and insulin sensitivity of rats fed high fructose diets
Vitamin E improves the free radical defense system potential and insulin sensitivity of rats fed high fructose diets
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DOI：
10.1093/jn/127.1.103
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发表时间：
1997-01-01
影响因子：
4.2
通讯作者：
Halimi, S
中科院分区：
文献类型：
--
作者：
Faure, P;Rossini, E;Halimi, S
本研究的目的是研究维生素E对高果糖饮食导致胰岛素抵抗的大鼠的影响，对自由基防御系统的某些成分和胰岛素敏感性的影响。老鼠(断奶,50 g)被分成三组:对照组(C、n = 16),获得纯化的饮食含60克/ 100克碳水化合物,那些吃了果糖的高集团(英国《金融时报》,n = 16),美联储56.8%的碳水化合物的饮食,果糖,和一个高果糖和维生素E-fed集团(FVE, n = 16),美联储的英国《金融时报》饮食补充维生素E 3.4克/公斤饮食(和0.17克/公斤在C和英国《金融时报》组),治疗的持续时间为6周。采用正糖-高胰岛素-葡萄糖钳夹技术测定各组中半数大鼠的胰岛素敏感性，其余大鼠测定血浆葡萄糖、胰岛素、甘油三酯和果糖胺浓度以及自由基防御系统成分。FT组胰岛素敏感性明显低于C组，各组间血糖水平无显著差异。与C组相比，FT组脂质过氧化程度更高，表现为血浆硫代巴比妥酸反应物质（TBARS）和血液二硫代谷胱甘肽（GSSG）浓度较高，Cu-Zn超氧化物歧化酶（Cu-Zn SOD）活性较低。此外，FVE组胰岛素敏感性高于FT组，但仍低于C组。这些结果表明，高果糖饮食导致大鼠胰岛素抵抗和自由基防御系统缺陷，补充维生素E可改善果糖喂养大鼠的胰岛素敏感性。
The purpose of this study was to investigate the effects of vitamin E in rats fed a high fructose diet which leads to insulin resistance, on some components of the free radical defense system and on insulin sensitivity. The rats (postweaning, 50 g) were divided into three groups: the control group (C, n = 16), which received a purified diet containing 60 g/100 g carbohydrates, the high fructose-fed group (FT, n = 16), fed a diet in which 56.8% of the carbohydrate as fructose, and a high fructose and vitamin E-fed group (FVE, n = 16), fed the FT diet supplemented with 3.4 g vitamin E/kg diet (vs. 0.17 g/kg in C and FT groups), The duration of the treatment was 6 wk. Insulin sensitivity was determined in half of the rats in each group using the euglycemic hyperinsulinic glucose clamp technique, The remaining rats were investigated for plasma glucose, insulin, triglyceride and fructosamine concentrations and for components of the free radical defense system. The FT group had a significantly lower insulin sensitivity than the C group, Basal glycemia was not different among the groups, In comparison with the C group, the FT group had a greater lipid peroxidation, as indicated by the higher concentrations of plasma thiobarbituric acid reactive substances (TBARS) and blood disulfide glutathione (GSSG) and the lower Cu-Zn superoxide dismutase (Cu-Zn SOD) activity. These markers approached the values of the controls after addition of vitamin E, Moreover, the FVE group had a higher insulin sensitivity than the FT group, but it remained lower than in the C group, These results show that a high fructose diet in rats leads to insulin resistance and a defect in the free radical defense system, Vitamin E supplementation improves insulin sensitivity in fructose-fed rats.