Variations in spatial and temporal distribution of Archaea in the North Sea in relation to environmental variables
Variations in spatial and temporal distribution of Archaea in the North Sea in relation to environmental variables
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DOI：
10.1111/j.1574-6941.2007.00397.x
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发表时间：
2007-12-01
影响因子：
4.2
通讯作者：
Damste, Jaap S. Sinninghe
中科院分区：
文献类型：
--
作者：
Herfort, Lydie;Schouten, Stefan;Damste, Jaap S. Sinninghe
通过rRNA杂交、16S rRNA基因测序和定量分析以及膜脂分析，结合物理、化学和生物学参数，研究了北海南部浮游古生物的时空分布，以确定影响古生物地理的因素。一个明确的时间变化，观察到海洋Crenarchaeota（组I.1a）是相对更丰富的冬季和Euryarchaeota占主导地位的古菌组合在春季和夏季。泉古菌横向分布的空间差异也很明显。事实上，它们的丰度与编码crenarchaeotal氨单加氧酶（amoA）α亚基的基因的拷贝数呈正相关，并与氨，硝酸盐，亚硝酸盐和磷的浓度呈正相关。这表明，大多数Crenarchaeota在北海是硝化菌，它们的分布是由营养浓度。然而，Crenarchaeota并不丰富时，较大的浮游植物（> 3 μ m）占主导地位的藻类种群。据推测，连同营养盐浓度，浮游植物生物量和群落结构可以预测crenarchaeotal丰度在北海南部。广古菌丰度与叶绿素a浓度呈正相关，但与浮游植物群落结构无关。这是否与Euryarchaeota进行有氧缺氧光养的潜力有关尚待证明，但Crenarchaeota和Euryarchaeota明显的季节分布模式表明它们占据了不同的生态位。
The spatial and temporal distribution of pelagic Archaea was studied in the southern North Sea by rRNA hybridization, sequencing and quantification of 16S rRNA gene and membrane lipid analyses and related to physical, chemical and biological parameters to determine the factors influencing archaeal biogeography. A clear temporal variability was observed, with marine Crenarchaeota (Group I.1a) being relatively more abundant in winter and Euryarchaeota dominating the archaeal assemblage in spring and summer. Spatial differences in the lateral distribution of Crenarchaeota were also evident. In fact, their abundance was positively correlated with the copy number of the gene encoding the alpha subunit of crenarchaeotal ammonia monooxygenase (amoA) and with concentrations of ammonia, nitrate, nitrite and phosphorus. This suggests that most Crenarchaeota in the North Sea are nitrifiers and that their distribution is determined by nutrient concentrations. However, Crenarchaeota were not abundant when larger phytoplankton (> 3 mu m) dominated the algal population. It is hypothesized that together with nutrient concentration, phytoplankton biomass and community structure can predict crenarchaeotal abundance in the southern North Sea. Euryarchaeotal abundance was positively correlated with chlorophyll a concentrations, but not with phytoplankton community structure. Whether this is related to the potential of Euryarchaeota to perform aerobic anoxygenic phototrophy remains to be shown, but the conspicuous seasonal distribution pattern of Crenarchaeota and Euryarchaeota suggests that they occupy a different ecological niche.