Evaluation of the nutrition and function of cow and goat milk based on intestinal microbiota by metagenomic analysis
Evaluation of the nutrition and function of cow and goat milk based on intestinal microbiota by metagenomic analysis
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基于肠道菌群的宏基因组分析评价牛羊奶的营养和功能
DOI：
10.1039/c7fo01780d
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发表时间：
2018
期刊：
影响因子：
6.1
通讯作者：
Zhan Jiachao
中科院分区：
文献类型：
--
作者：
Wang Zhaoxia;Jiang Shuaiming;Ma Chenchen;Huo Dongxue;Peng Qiannan;Shao Yuyu;Zhan Jiachao
牛奶中含有人体所需的营养物质，不同动物牛奶的主要成分各不相同。因此，我们使用高通量16S rRNA测序技术评估了奶牛和羊奶的营养及其对小鼠肠道微生物群的影响。饲喂牛奶和羊奶后，小鼠肠道菌群发生显著变化，羊奶对肠道菌群的影响大于牛奶。与对照组相比，牛奶组和羊奶组双歧杆菌、Allobaculum、Olsenella和Akkermansia均显著增加，说明牛奶对其生长有积极影响。我们还发现，羊奶组的柠檬酸循环（TCA循环）、丙酮酸代谢、氨基糖和核苷酸糖代谢以及硫辛酸代谢均高于牛奶组。乳成分及其代表性肠道菌群相关性分析显示，酪蛋白、αs1-酪蛋白和β + κ-酪蛋白与肠球菌和异源菌呈正相关，与玫瑰菌呈负相关。蛋白和αs2-酪蛋白与Akkermansia、双歧杆菌和真杆菌呈正相关。
Milk contains nutrients needed by the body, and the main components of different animal milk vary. Accordingly, we evaluated cow and goat milk's nutrition and their effects on the gut microbiota in mice models using a high-throughput 16S rRNA sequencing technology. The intestinal microbiota of mice changed significantly after the intake of cow and goat milk, and the goat milk had a greater effect on the intestinal microbial community than the cow milk. Bifidobacterium, Allobaculum, Olsenella and Akkermansia grew significantly in both cow and goat milk groups compared with the control group, indicating that milk positively affected their growth. We also found that the citrate cycle (TCA cycle), pyruvate metabolism, and amino sugar and nucleotide sugar metabolism, as well as lipoic acid metabolism, were higher in the goat milk group than in the cow milk group. Association analysis of milk components and their representative intestinal microbiota showed that casein, αs1-casein, and β + κ-casein were positively correlated with Enterococcus and Allobaculum, and negatively correlated with Roseburia. Protein and αs2-casein were positively associated with Akkermansia, Bifidobacterium and Eubacterium.