Significance of Enhanced Expression of Nitric Oxide Syntheses in Splenic Sinus Lining Cells in Altered Portal Hemodynamics of Idiopathic Portal Hypertension
Significance of Enhanced Expression of Nitric Oxide Syntheses in Splenic Sinus Lining Cells in Altered Portal Hemodynamics of Idiopathic Portal Hypertension
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DOI：
10.1007/s10620-006-9146-9
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发表时间：
2007-04
影响因子：
3.1
通讯作者：
Yasunori Sato;S. Sawada;K. Kozaka;K. Harada;M. Sasaki;O. Matsui;Y. Nakanuma
中科院分区：
文献类型：
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作者：
Yasunori Sato;S. Sawada;K. Kozaka;K. Harada;M. Sasaki;O. Matsui;Y. Nakanuma
特发性门静脉高压（IPH）以非肝硬化门静脉高压为特征，主要是由于肝内、窦前对门静脉血流的抵抗增加。脾肿大常见于IPH。为明确脾肿大的病理意义，采用免疫组化方法检测IPH患者脾组织中诱导型一氧化氮合成（iNOS）、内皮型一氧化氮合成（eNOS）和内皮素-1 （ET-1）的表达。IPH脾窦壁细胞呈弥漫性强表达iNOS和eNOS。肝硬化（LC）患者脾脏窦壁细胞iNOS和eNOS也呈阳性，但染色强度明显减弱。在IPH和LC脾脏的红髓中，只有少数单核白细胞检测到ET-1。这些结果表明，脾脏释放的NO，而不是ET-1，负责脾窦扩张，导致脾肿大，从而导致IPH的门静脉高压。
Idiopathic portal hypertension (IPH) is characterized by noncirrhotic portal hypertension due mainly to increased intrahepatic, presinusoidal resistance to portal blood flow. Marked splenomegaly is always seen in IPH. To clarify the pathogenetic significance of splenomegaly, immunohistochemical expression of inducible nitric oxide synthese (iNOS), endothelial NOS (eNOS), and endothelin-1 (ET-1) in spleens from patients with IPH was examined. Sinus lining cells of IPH spleens showed diffuse and strong expression of iNOS and eNOS. Sinus lining cells of spleens from patients with liver cirrhosis (LC) also showed positive signals for iNOS and eNOS, but the staining intensity was significantly weak. ET-1 was detectable in only a few mononuclear leukocytes in the red pulp of both IPH and LC spleens. These results suggest that NO liberated in spleen, rather than ET-1, is responsible for the dilatation of splenic sinuses, leading to splenomegaly, and thereby contributes to portal hypertension in IPH.