Biological methane production coupled with sulfur oxidation in a microbial electrosynthesis system without organic substrates
Biological methane production coupled with sulfur oxidation in a microbial electrosynthesis system without organic substrates
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DOI：
10.1016/j.jes.2021.07.027
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发表时间：
2022-01-11
影响因子：
6.9
通讯作者：
Ohashi, Akiyoshi
中科院分区：
文献类型：
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作者：
Dinh, Ha T. T.;Kambara, Hiromi;Ohashi, Akiyoshi
甲烷是在没有有机底物的微生物电合成系统（MES）中产生的。然而，生物电反应需要相对较高的施加电压。在这项研究中，我们证明即使在 MES 中 0.1 V 的极低电压下，也可以在生物阴极实现电养甲烷生产，其中非生物 HS(-) 在涂有铂粉的阳极碳布表面氧化为 SO42-。此外，微生物群落分析揭示了从电子产生甲烷的最可能途径。首先，电养型H-2是由合养细菌（例如合养杆菌属、合养杆菌属、合养菌属、细线菌属和氨基菌目）产生的，在生物阴极直接接受电子。随后，大部分产生的 H-2 被同型产乙酸菌（如梭状芽孢杆菌和螺旋体 2）转化为乙酸盐。总之，大部分甲烷是由大量的乙酸分解产甲烷菌（即甲烷菌）通过乙酸盐间接产生的。此外，氢营养产甲烷菌，包括甲烷杆菌和产甲烷菌，通过H-2产生甲烷。 (C) 2022 中国科学院生态环境研究中心。由 Elsevier B.V. 出版
Methane is produced in a microbial electrosynthesis system (MES) without organic sub-strates. However, a relatively high applied voltage is required for the bioelectrical reactions. In this study, we demonstrated that electrotrophic methane production at the biocathode was achieved even at a very low voltage of 0.1 V in an MES, in which abiotic HS(-)oxidized to SO42- at the anodic carbon-cloth surface coated with platinum powder. In addition, microbial community analysis revealed the most probable pathway for methane production from electrons. First, electrotrophic H-2 was produced by syntrophic bacteria, such as Syntrophorhabdus, Syntrophobacter, Syntrophus, Leptolinea, and Aminicenantales, with the direct acceptance of electrons at the biocathode. Subsequently, most of the produced H-2 was converted to acetate by homoacetogens, such as Clostridium and Spirochaeta 2. In conclusion, the majority of the methane was indirectly produced by a large population of acetoclastic methanogens, namely Methanosaeta, via acetate. Further, hydrogenotrophic methanogens, including Methanobacterium and Methanolinea, produced methane via H-2. (C) 2022 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.