Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament
Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament
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DOI：
10.1038/nsb901
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发表时间：
2003-03-01
期刊：
影响因子：
--
通讯作者：
Kowalczykowski, SC
中科院分区：
文献类型：
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作者：
Alexeev, A;Mazin, A;Kowalczykowski, SC
在酿酒酵母中，Rad54蛋白与Rad51、Rad52、Rad55和Rad57蛋白一起参与双链DNA断裂的重组修复。在体外，Rad54与Rad51相互作用并刺激Rad51蛋白促进的DNA链交换。Rad54是一种SWI2/SNF2相关蛋白，具有双链DNA依赖的ATP酶活性，并以ATP水解依赖的方式改变DNA拓扑结构。在这里，我们证明了Rad54通过沿DNA滑动核小体来催化核小体的双向再分配。Rad51核蛋白丝极大地刺激了核小体的再分配，但不需要在丝内存在同源单链DNA。在这些数据的基础上，我们提出Rad54促进染色质重塑，也许更广泛地说，在基因重组的同源性搜索步骤中，蛋白质清除。
In Saccharomyces cerevisiae, the Rad54 protein participates in the recombinational repair of double-strand DNA breaks together with the Rad51, Rad52, Rad55 and Rad57 proteins. In vitro, Rad54 interacts with Rad51 and stimulates DNA strand exchange promoted by Rad51 protein. Rad54 is a SWI2/SNF2- related protein that possesses double-stranded DNA-dependent ATPase activity and changes DNA topology in an ATP hydrolysis-dependent manner. Here we show that Rad54 catalyzes bidirectional nucleosome redistribution by sliding nucleosomes along DNA. Nucleosome redistribution is greatly stimulated by the Rad51 nucleoprotein filament but does not require the presence of homologous single-stranded DNA within the filament. On the basis of these data, we propose that Rad54 facilitates chromatin remodeling and, perhaps more generally, protein clearing at the homology search step of genetic recombination.