Virus-Encapsulated DNA Origami Nanostructures for Cellular Delivery
Virus-Encapsulated DNA Origami Nanostructures for Cellular Delivery
复制标题
DOI：
10.1021/nl500677j
复制
发表时间：
2014-04-01
期刊：
影响因子：
10.8
通讯作者：
Kostiainen, Mauri A.
中科院分区：
文献类型：
--
作者：
Mikkila, Joona;Eskelinen, Antti-Pekka;Kostiainen, Mauri A.
DNA折纸结构可以被编程为具有纳米级精度的任意形状，这为设计新型功能材料开辟了许多有吸引力的机会。一个有趣的可能性是使用DNA origamis进行完全可调的，靶向的和触发的药物递送。在这项工作中，我们证明了涂层的DNA折纸纳米结构与病毒衣壳蛋白，以提高细胞的交付。我们的方法利用纯化的豇豆褪绿斑驳病毒衣壳蛋白，可以通过静电相互作用在折纸表面结合和自组装，并进一步将折纸纳米结构包装在病毒衣壳内。用人HEK293细胞系进行的共聚焦显微镜成像和转染研究表明，与裸DNA origamis相比，蛋白质涂层改善了细胞附着和origamis向细胞中的递送13倍。所提出的方法可以很容易地找到应用程序，不仅在复杂的药物输送应用程序，但也在组织细胞内反应折纸为基础的模板。
DNA origami structures can be programmed into arbitrary shapes with nanometer scale precision, which opens up numerous attractive opportunities to engineer novel functional materials. One intriguing possibility is to use DNA origamis for fully tunable, targeted, and triggered drug delivery. In this work, we demonstrate the coating of DNA origami nanostructures with virus capsid proteins for enhancing cellular delivery. Our approach utilizes purified cowpea chlorotic mottle virus capsid proteins that can bind and self-assemble on the origami surface through electrostaticinteractions and further pack the origami nanostructures inside the viral capsid. Confocal microscopy imaging and transfection studies with a human HEK293 cell line indicate that protein coating improves cellular attachment and delivery of origamis into the cells by 13-fold compared to bare DNA origamis. The presented method could readily find applications not only in sophisticated drug delivery applications but also in organizing intracellular reactions by origami-based templates.