USE OF AUTOMATED SEQUENCING OF POLYMERASE CHAIN REACTION-GENERATED AMPLICONS TO IDENTIFY 3 TYPES OF CHOLERA-TOXIN SUBUNIT-B IN VIBRIO-CHOLERAE O1 STRAINS
USE OF AUTOMATED SEQUENCING OF POLYMERASE CHAIN REACTION-GENERATED AMPLICONS TO IDENTIFY 3 TYPES OF CHOLERA-TOXIN SUBUNIT-B IN VIBRIO-CHOLERAE O1 STRAINS
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DOI：
10.1128/jcm.31.1.22-25.1993
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发表时间：
1993-01-01
影响因子：
9.4
通讯作者：
FIELDS, PI
中科院分区：
文献类型：
--
作者：
OLSVIK, O;WAHLBERG, J;FIELDS, PI
霍乱毒素是引起大量肠液分泌的主要因素，这是霍乱的特征。通过对聚合酶链反应产生的扩增子进行自动DNA测序，确定了70年来从29个国家分离的45株霍乱弧菌01菌株的霍乱毒素亚单位B结构基因的DNA序列。霍乱毒素B亚单位基因（ctx B）有三种类型。基因型1发现于全球经典生物型菌株和与美国墨西哥湾沿岸相关的El Tor生物型菌株中，基因型2发现于来自澳大利亚的El Tor生物型菌株中，基因型3发现于来自第七次大流行和最近拉丁美洲流行的El Tor生物型菌株中。所有的碱基变化都对应于霍乱毒素B亚单位中的氨基酸取代。B亚单位的异质性可能对霍乱毒素和抗毒素的疫苗开发和诊断试验有意义。我们的结论是，这项技术提供了及时和潜在有用的流行病学信息。
Cholera toxin is the principal factor causing the profuse intestinal fluid secretion that is characteristic of cholera. The DNA sequences of the cholera toxin subunit B structural genes from 45 Vibrio cholerae 01 strains isolated in 29 countries over a period of 70 years were determined by automated DNA sequencing of polymerase chain reaction-generated amplicons. Three types of cholera toxin B subunit gene (ctxB) were identified. Genotype 1 was found in strains of classical biotype worldwide and El Tor biotype strains associated with the U.S. Gulf Coast, genotype 2 was found in El Tor biotype strains from Australia, and genotype 3 was found in El Tor biotype strains from the seventh pandemic and the recent Latin American epidemic. All base changes correspond to an amino acid substitution in the B subunit of the cholera toxin. Heterogenicity in the B subunit could have implications for vaccine development and diagnostic tests for cholera toxin and antitoxin. We conclude that this technology provides timely and potentially useful epidemiological information.