THE PRIMARY STRUCTURE AND EXPRESSION OF THE 2ND OPEN READING FRAME OF THE POLYMERASE GENE OF THE CORONAVIRUS MHV-A59 - A HIGHLY CONSERVED POLYMERASE IS EXPRESSED BY AN EFFICIENT RIBOSOMAL FRAMESHIFTING MECHANISM
THE PRIMARY STRUCTURE AND EXPRESSION OF THE 2ND OPEN READING FRAME OF THE POLYMERASE GENE OF THE CORONAVIRUS MHV-A59 - A HIGHLY CONSERVED POLYMERASE IS EXPRESSED BY AN EFFICIENT RIBOSOMAL FRAMESHIFTING MECHANISM
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DOI：
10.1093/nar/18.7.1825
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发表时间：
1990-04-11
影响因子：
14.9
通讯作者：
SPAAN, WJM
中科院分区：
文献类型：
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作者：
BREDENBEEK, PJ;PACHUK, CJ;SPAAN, WJM
对鼠冠状病毒MHV-A59的聚合酶基因的大部分进行的序列分析显示，开放阅读框（ORE 1a）的3“端与覆盖聚合酶基因3”半的大ORF（ORF 1b; 2733个氨基酸）重叠。ORF 1b的表达通过核糖体移码机制发生，因为ORF 1a/ORF 1b重叠核苷酸序列能够在体外和体内诱导核糖体移码。一个茎环结构和一个假结预测在核糖体移码的核苷酸序列参与。将预测的MHV ORF 1b的氨基酸序列与禽冠状病毒IBV相应基因推导的氨基酸序列进行比较，表明与其他病毒基因相比，该ORF非常保守。预测的氨基酸序列的详细分析揭示了在许多DNA和RNA聚合酶中保守的序列元件。
Sequence analysis of a substantial part of the polymerase gene of the murine coronavirus MHV-A59 revealed the 3'' end of an open reading frame (ORE1a) overlapping with a large ORF (ORF1b; 2733 amino acids) which covers the 3'' half of the polymerase gene. The expression of ORF1b occurs by a ribosomal frameshifting mechanism since the ORF1a/ORF1b overlapping nucleotide sequence is capable of inducing ribosomal frameshifting in vitro as well as in vivo. A stem-loop structure and a pseudoknot are predicted in the nucleotide sequence involved in ribosomal frameshifting. Comparison of the predicted amino acid sequence of MHV ORF1b with the amino acid sequence deduced from the corresponding gene of the avian coronavirus IBV demonstrated that in contrast to the other viral genes this ORF is extremely conserved. Detailed analysis of the predicted amino acid sequence revealed sequence elements which are conserved in many DNA and RNA polymerases.