Evolution of the dorsal-ventral patterning network in the mosquito, Anopheles gambiae
Evolution of the dorsal-ventral patterning network in the mosquito, Anopheles gambiae
复制标题
DOI：
10.1242/dev.02863
复制
发表时间：
2007-07-01
期刊：
影响因子：
4.6
通讯作者：
Levine, Mike
中科院分区：
文献类型：
--
作者：
Goltsev, Yury;Fuse, Naoyuki;Levine, Mike
果蝇胚胎的背-腹模式是由一个定义良好的基因调控网络控制的。我们希望了解这个网络的变化如何在昆虫原肠胚形成中产生进化多样性。本研究的重点是两个高度分化的双翅目昆虫，果蝇黑腹果蝇和冈比亚按蚊的背外胚层。在黑腹龙中，背外胚层的背中线形成一个单一的胚外膜，即羊膜。在冈比亚疟蚊中，一个扩展的结构域形成了两个不同的胚胎外组织，羊膜和浆膜。对大约20种不同背-腹侧模式基因的分析表明，果蝇和蚊子的中胚层和腹侧神经源性外胚层的初始特征高度保守。相比之下，在背外胚层中活跃的基因的表达谱有许多差异。最值得注意的是，冈比亚拟南芥胚胎外结构域细分为单独的羊膜和浆膜谱系与几种片段抑制因子的新基因表达模式相关。此外，与D. melanogaster胚胎相比，扩大的羊膜和浆膜斑块与更广泛的Dpp信号相关。有证据表明，这种扩大的信号是由于sog基因表达的改变。
The dorsal-ventral patterning of the Drosophila embryo is controlled by a well-defined gene regulation network. We wish to understand how changes in this network produce evolutionary diversity in insect gastrulation. The present study focuses on the dorsal ectoderm in two highly divergent dipterans, the fruitfly Drosophila melanogaster and the mosquito Anopheles gambiae. In D. melanogaster, the dorsal midline of the dorsal ectoderm forms a single extra-embryonic membrane, the amnioserosa. In A. gambiae, an expanded domain forms two distinct extra-embryonic tissues, the amnion and serosa. The analysis of approximately 20 different dorsal-ventral patterning genes suggests that the initial specification of the mesoderm and ventral neurogenic ectoderm is highly conserved in flies and mosquitoes. By contrast, there are numerous differences in the expression profiles of genes active in the dorsal ectoderm. Most notably, the subdivision of the extra-embryonic domain into separate amnion and serosa lineages in A. gambiae correlates with novel patterns of gene expression for several segmentation repressors. Moreover, the expanded amnion and serosa anlage correlates with a broader domain of Dpp signaling as compared with the D. melanogaster embryo. Evidence is presented that this expanded signaling is due to altered expression of the sog gene.