Lines of Evidence for Horizontal Gene Transfer of a Phenazine Producing Operon into Multiple Bacterial Species
Lines of Evidence for Horizontal Gene Transfer of a Phenazine Producing Operon into Multiple Bacterial Species
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DOI：
10.1007/s00239-009-9198-5
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发表时间：
2009-02-01
影响因子：
3.9
通讯作者：
Fitzpatrick, David A.
中科院分区：
文献类型：
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作者：
Fitzpatrick, David A.
吩嗪类化合物是一种具有广谱抗菌活性的次生代谢物，对细菌、真菌和真核生物具有抗菌活性。在假单胞菌中，需要一个保守的七基因吩嗪操纵子(PhzABCDEFG)来将分枝酸转化为广谱抗生素吩嗪-1-羧酸盐。以前对非假单胞菌产生吩嗪的基因的分析发现，与假单胞菌同源物有很高的序列相似性。这项研究中进行的分析希望阐明与吩嗪类药物生产有关的基因的进化史。此外，我想确定是否通过水平基因转移转移了吩嗪操纵子。通过GC含量、密码子使用模式、3：1二核苷酸频率、序列相似性和系统发育重建的分析，绘制了来自多个细菌物种的吩嗪基因的进化历史。斑块状的系统发育分布、高度的序列相似性和系统发育证据推测，假单胞菌、肉桂链霉菌、聚集性泛胞杆菌、洋葱伯克霍尔德氏菌、腐烂杆菌、亚麻短杆菌和脓肿分枝杆菌都含有吩嗪操纵子，很可能是通过水平基因转移在这些物种之间转移的。获得与抗生素相关的操纵子意义重大，因为它可以增加受体物种的相对适合度。
Phenazines are secondary metabolites with broad-spectrum antibiotic activity against bacteria, fungi, and eukaryotes. In pseudomonad species, a conserved seven-gene phenazine operon (phzABCDEFG) is required for the conversion of chorismic acid to the broad-spectrum antibiotic phenazine-1-carboxylate. Previous analyses of genes involved in phenazine production from nonpseudomonad species uncovered a high degree of sequence similarity to pseudomonad homologues. The analyses undertaken in this study wished to eluciadate the evolutionary history of genes involved in the production of phenazines. Furthermore, I wanted to determine if the phenazine operon has been transferred through horizontal gene transfer. Analyses of GC content, codon usage patterns, frequency of 3:1 dinucleotides, sequence similarities, and phylogenetic reconstructions were undertaken to map the evolutionary history of phenazine genes from multiple bacterial species. Patchy phyletic distribution, high sequence similarities, and phylogenetic evidence infer that pseudomonad, Streptomyces cinnamonensis, Pantoea agglomerans, Burkholderia cepacia, Pectobacterium atrosepticum, Brevibacterium linens, and Mycobacterium abscessus species all contain a phenazine operon which has most likely been transferred among these species through horizontal gene transfer. The acquisition of an antibiotic-associated operon is significant, as it may increase the relative fitness of the recipient species.