Bacteriophage P22 and Staphylococcus aureus attenuation on nonporous fomites as determined by plate assay and quantitative PCR

Bacteriophage P22 and Staphylococcus aureus attenuation on nonporous fomites as determined by plate assay and quantitative PCR
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DOI:
10.1128/aem.00352-08
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发表时间:
2008-09-01
影响因子:
4.4
通讯作者:
Rose, Joan B.
Rose, Joan B.
中科院分区:
生物学2区
文献类型:
--
作者:
Masago, Yoshifumi;Shibata, Tomoyuki;Rose, Joan B.

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基于培养和定量 PCR 评估了噬菌体 P22 和金黄色葡萄球菌在六种常见家庭无生命表面上的腐烂率。使用平板测定观察到更高水平的灭活,这表明在污染物样本中检测到病原体基因组并不一定意味着对人类存在健康风险。
Decay rates of bacteriophage P22 and Staphylococcus aureus on six types of common household inanimate surfaces were evaluated based on cultivation and quantitative PCR. A much higher level of inactivation was observed using the plate assay, suggesting that detection of the pathogen genome in samples from fomites does not necessarily imply a health risk to humans.