Critical roles of mitochondria in brain activities of torpid Myotis ricketti bats revealed by a proteomic approach
Critical roles of mitochondria in brain activities of torpid Myotis ricketti bats revealed by a proteomic approach
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蛋白质组学方法揭示线粒体在呆滞立氏鼠耳蝠大脑活动中的关键作用
DOI：
10.1016/j.jprot.2014.01.006
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发表时间：
2014-06-13
影响因子：
3.3
通讯作者：
Zhang, Shuyi
中科院分区：
文献类型：
--
作者：
Zhang, Yijian;Pan, Yi-Hsuan;Zhang, Shuyi
蝙蝠是唯一会飞行和冬眠的哺乳动物。人们对冬眠期间大脑中的整体代谢知之甚少。在这项研究中，使用蛋白质组学方法对呆滞和活跃的立氏鼠耳蝠的大脑蛋白质进行了分级和比较。结果显示，21%（23 个蛋白质）具有显着表达变化的已识别蛋白质与氨基酸代谢和蛋白质稳态相关。在迟钝的蝙蝠中，参与能量代谢（15 种蛋白质）、细胞骨架结构（18 种蛋白质）和应激反应（13 种蛋白质）的蛋白质的表达水平也发生了显着改变。超过 30%（34 种蛋白质）的差异表达蛋白质与线粒体功能相关。检测到 PDHB、DLD 和 ARG1 上的各种翻译后修饰 (PTM)，表明蝙蝠在休眠期间利用 PTM 来调节蛋白质功能。迟钝的蝙蝠的抗氧化和应激反应与冬眠的松鼠相似，这表明小型冬眠动物对抗大脑功能障碍所采取的共同策略。由于线粒体中代谢的许多氨基酸调节神经元传递，因此这项研究的结果揭示了线粒体在蝙蝠冬眠期间的神经通讯、代谢调节和脑细胞存活中的关键作用。本文是题为“非模式生物的蛋白质组学”的特刊的一部分。生物学意义这项研究揭示了蝙蝠在冬眠期间调节大脑活动的机制。这些机制包括参与线粒体电子传递、无氧糖酵解、TCA循环流出、细胞骨架可塑性、氨基酸代谢、囊泡结构、抗氧化防御、应激反应和蛋白质稳态的蛋白质的翻译后修饰和差异表达。我们的研究提供了飞行哺乳动物在冬眠期间的代谢调节以及小型冬眠者应对冬眠的常见策略的见解。本文是题为“非模式生物的蛋白质组学”的特刊的一部分。 (C) 2014 Elsevier B.V. 保留所有权利。
Bats are the only mammals that fly and hibernate. Little is known about their overall metabolism in the brain during hibernation. In this study, brain proteins of torpid and active Myotis ricketti bats were fractionated and compared using a proteomic approach. Results showed that 21% (23 proteins) of identified proteins with significant expression changes were associated with amino acid metabolism and proteostasis. The expression levels of proteins involved in energy metabolism (15 proteins), cytoskeletal structure (18 proteins), and stress response (13 proteins) were also significantly altered in torpid bats. Over 30% (34 proteins) of differentially expressed proteins were associated with mitochondrial functions. Various post-translational modifications (PTMs) on PDHB, DLD, and ARG1 were detected, suggesting that bats use PTMs to regulate protein functions during torpor. Antioxidation and stress responses in torpid bats were similar to those of hibernated squirrels, suggesting a common strategy adopted by small hibernators against brain dysfunction. Since many amino acids that metabolize in mitochondria modulate neuronal transmissions, results of this study reveal pivotal roles of mitochondria in neural communication, metabolic regulation, and brain cell survival during bat hibernation. This article is part of a Special Issue entitled: Proteomics of non-model organisms.Biological significanceThis study reveals the mechanisms used by bats to regulate brain activities during torpor. These mechanisms include post-translational modifications and differential expression of proteins involved in mitochondrial electron transport, anaerobic glycolysis, TCA cycle efflux, cytoskeletal plasticity, amino acid metabolism, vesicle structure, antioxidation defense, stress response, and proteostasis. Our study provides insights in metabolic regulation of flying mammals during torpor and common strategies used by small hibernators in response to hibernation.This article is part of a Special Issue entitled: Proteomics of non-model organisms. (C) 2014 Elsevier B.V. All rights reserved.