A Novel Interaction between Perlecan Protein Core and Progranulin
A Novel Interaction between Perlecan Protein Core and Progranulin
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基底膜蛋白核心和颗粒体蛋白前体之间的新型相互作用
DOI：
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发表时间：
2003
影响因子：
4.8
通讯作者：
R. Iozzo
中科院分区：
文献类型：
--
作者：
Eva M. Gonzalez;M. Mongiat;S. Slater;R. Baffa;R. Iozzo
In an in vivo search of novel partners for perlecan, a major heparan sulfate proteoglycan of basement membranes and cell surfaces, we identified progranulin, a secreted growth factor, as a strong interacting protein. Unambiguous interaction, first observed with the yeast two-hybrid system, was corroborated by co-immunoprecipitation studies using cell-free transcription/translation and transient cell transfection assays. The interaction of progranulin with perlecan domain V involved the first two laminin- and epidermal growth factor-like repeats. Within progranulin, the subdomains interacting most with perlecan harbored granulins F and B. Kinetics analysis of the interaction using surface plasmon resonance showed a saturable binding of relative low affinity (KD ∼1 μm). These results were supported by significant expression overlap of these two proteins in a series of ovarian tumor tissue microarrays. Progranulin was present within proliferating blood vessels of ovarian carcinomas and perivascular matrices, with a distribution similar to perlecan. Notably, both progranulin and domain V stimulated the growth of adrenal carcinoma cells. However, when used together in equimolar amounts, the two proteins counteracted each other's activity. Thus, progranulin/perlecan interaction could contribute to a fine regulation of tumor angiogenesis and could ultimately affect cancer growth.
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转移性黑色素瘤中基底膜蛋白聚糖的异常表达。
影响因子：
11.2
作者：
Cohen,IR;Murdoch,AD;Naso,MF;Marchetti,D;Berd,D;Iozzo,RV
通讯作者：
Iozzo,RV
通过针对结构域 III 的新型单克隆抗体和原位杂交检测到基底膜蛋白多糖在人体组织的基底膜和细胞外基质中的广泛表达。
DOI：
10.1177/42.2.7507142
发表时间：
1994
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Murdoch,AD;Liu,B;Schwarting,R;Tuan,RS;Iozzo,RV
通讯作者：
Iozzo,RV
DOI：
10.1016/s0021-9258(18)82064-6
发表时间：
1993-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jian Zhou;G. Gao;G. Gao;J. Crabb;G. Serrero
通讯作者：
Jian Zhou;G. Gao;G. Gao;J. Crabb;G. Serrero
影响因子：
15.9
作者：
Sharma, B;Handler, M;Iozzo, RV
通讯作者：
Iozzo, RV
影响因子：
4.7
作者：
Jones, MB;Spooner, M;Kohn, EC
通讯作者：
Kohn, EC