Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
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DOI：
10.3109/10520295.2012.717304
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发表时间：
2013-01-01
影响因子：
1.6
通讯作者：
Vozari-Hampe, M. M.
中科院分区：
文献类型：
--
作者：
Dresch, R. R.;Zanetti, G. D.;Vozari-Hampe, M. M.
瓦楞纸轴菌凝集素1 （ACL-1）是从海绵瓦楞纸轴菌水提物中纯化得到的。ACL-1能强烈凝集家兔红细胞。N-乙酰基衍生物，特别是N， N‘， N’ -三乙酰壳聚糖、N-乙酰- d -葡萄糖胺、N-乙酰- d -甘露糖胺和N-乙酰- d -半乳糖胺能抑制血凝。我们研究了生物素化的ACL-1对几种转化细胞系的染色能力，包括乳腺（T-47D, MCF7）、结肠（HT-29）、肺（H460）、卵巢（OVCAR-3）和膀胱（T24）。ACL-I可以结合肿瘤细胞的单糖和寡糖、n -乙酰- d -半乳糖胺和n -乙酰- d -氨基葡萄糖聚糖类型。凝集素是有用的，不仅作为标志物和诊断参数，而且对可疑肿瘤的组织定位。此外，它们在细胞学和分子水平上提供了对肿瘤更好的理解。此外，使用潜在的转移标志物，如凝集素，对于开发成功的抗癌治疗工具至关重要。我们观察到，生物素化的acl - 1染色肿瘤细胞，可能具有作为鉴定转化细胞和研究这些细胞合成的聚糖结构的探针的潜力。
Axinella corrugata lectin 1 (ACL-1) was purified from aqueous extracts of the marine sponge, Axinella corrugata. ACL-1 strongly agglutinates native rabbit erythrocytes. The hemagglutination is inhibited by N-acetyl derivatives, particularly N, N', N"-triacetylchitotriose, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine and N-acetyl-D-galactosamine. We investigated the capacity of biotinylated ACL-1 to stain several transformed cell lines including breast (T-47D, MCF7), colon (HT-29), lung (H460), ovary (OVCAR-3) and bladder (T24). ACL-I may bind to both monosaccharides and oligosaccharides of tumor cells, N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine glycan types. The lectins are useful, not only as markers and diagnostic parameters, but also for tissue mapping in suspicious neoplasms. In addition, they provide a better understanding of neoplasms at the cytological and molecular levels. Furthermore, the use of potential metastatic markers such as lectins is crucial for developing successful tools for therapy against cancer. We observed that biotinylated ACL-I stains tumor cells and may hold potential as a probe for identifying transformed cells and for studying glycan structures synthesized by such cells.