Evidence for the Translocation of Gibberellin A3 and Gibberellin-Like Substances in Grafts between Normal, Dwarf1 and Dwarf5 Seedlings of Zea mays L.
Evidence for the Translocation of Gibberellin A3 and Gibberellin-Like Substances in Grafts between Normal, Dwarf1 and Dwarf5 Seedlings of Zea mays L.
复制标题
玉米正常、矮化 1 和矮化 5 幼苗嫁接物中赤霉素 A3 和类赤霉素物质易位的证据。
DOI：
10.1093/oxfordjournals.pcp.a076527
复制
发表时间：
1983
影响因子：
4.9
通讯作者：
B. Phinney
中科院分区：
文献类型：
--
作者：
M. Katsumi;D. Foard;B. Phinney
在玉米正常苗和矮化苗的中胚轴切面之间进行了近接嫁接。(玉米)。矮秆为非等位单基因赤霉素突变体，矮化1和矮化5。嫁接组合为正常-正常、正常-矮化1、正常-矮化5、矮化-矮化1、矮化5-矮化5、矮化1-矮化5。这些移植物被用来展示赤霉素类物质在联盟中的移动。将GA3添加到嫁接的一个成员中，导致两个成员的叶鞘伸长。在正常-矮化1和正常-矮化5嫁接的矮化1和矮化5构件中观察到赤霉素样伸长。在某些情况下，在矮化1-矮化5嫁接的矮化1和矮化5中观察到伸长，尤其是在矮化5中。从玉米赤霉素的生物合成途径、d5和d1突变基因对该途径的控制以及类赤霉素物质在植物中的运动等方面对这一结果进行了讨论。共振后对嫁接苗的解剖观察表明，愈伤组织细胞发育并桥接在自体移植物的界面上。也有证据表明移植物愈合处存在血管分化。
Approach grafts were made between the cut surfaces of mesocotyls from normal and dwarf seedlings ofZea maysL. (maize). The dwarfs were the non-allelic single gene gibberellin mutants, dwarf1and dwarf5. The graft combinations were normal-normal, normal-dwarf1, normal-dwarf5, dwarfi-dwarf1, dwarf5-dwarf5, and dwarf1-dwarf5. The grafts were used to demonstrate the movement of gibberellin-like substances across the union. GA3, added to one member of the graft, resulted in leaf-sheath elongation to both members. Gibberellin-like elongation was observed in the dwarf1and dwarf5members of normal-dwarf1and normal-dwarf5grafts. Also, in some cases, elongation was observed in the dwarf1and dwarf5members of dwarf1-dwarf5grafts, especially in the dwarf5members. The results are discussed in terms of: the gibberellin biosynthetic pathway in maize, the control of this pathway by the d5and d1mutant genes, and the movement of gibberellin-like substances in the plant. Anatomical observation of grafted seedlings after the resonse was observed shows that callus cells developed and bridged across the interface of the autografts. There was also evidence for vascular differentiation across the graft union.