The use of a non-ionic surfactant (P188) to save chondrocytes from necrosis following impact loading of chondral explants
The use of a non-ionic surfactant (P188) to save chondrocytes from necrosis following impact loading of chondral explants
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DOI：
10.1016/j.orthres.2004.02.002
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发表时间：
2004-09-01
影响因子：
2.8
通讯作者：
Haut, RC
中科院分区：
文献类型：
--
作者：
Phillips, DM;Haut, RC
虽然目前汽车行业的损伤标准是基于骨折，但每年在碰撞中遭受的大多数膝盖损伤并不涉及骨折。相反，创伤性关节损伤的临床研究经常记录早期疼痛和慢性疾病的发展，如骨关节炎。先前的研究表明，关节机械损伤时关节软骨细胞死亡可引发慢性疾病。在目前的研究中，研究了软骨外植体钝性损伤后软骨细胞的早期坏死。一种非离子表面活性剂（P188）被认为是早期干预疾病过程的潜在工具，因为这种表面活性剂已被证明可以修复其他细胞系受损的膜。将三组成年牛软骨外植体在培养基中平衡培养48 h。两组在无侧限压缩下加载至25mpa。各组各取一半标本，上样后立即在添加8mg /ml p188的培养基中孵育，另一半返回标准培养基。在1和24小时用细胞活力染色法测定压缩组和对照组活细胞和死细胞的百分比。在创伤后1小时，P188孵育的标本与无P188组相比，浅表区活细胞百分比显著增加。24 h时，p188处理过的外植体三个区域的活细胞百分比均显著高于未处理组。本研究表明，P188表面活性剂可促进钝性机械损伤软骨的细胞膜完整性恢复。鉴于P188在体外软骨外体中“拯救”软骨细胞免于早期坏死死亡的能力，其在预防腹泻关节创伤后骨关节炎中的作用应在体内动物模型中进一步探索。(C) 2004骨科研究学会。Elsevier Ltd.出版。版权所有。
While current injury criteria for the automotive industry are based on bone fracture, the majority of knee injuries suffered in collisions each year do not involve fracture of bone. Instead, clinical studies of traumatic joint injury often document early pain and development of chronic diseases, such as osteoarthritis. Previous studies suggest chronic disease can be initiated by cell death that occurs in articular cartilage during mechanical trauma to the joint. In the current investigation early necrosis of chondrocytes was investigated after blunt trauma to chondral explants. A non-ionic surfactant (P188) was explored as a potential tool for early intervention into the disease process, as this surfactant has been shown to repair damaged membranes in other cell lines. Three groups of adult bovine chondral explants were equilibrated for 48 h in culture media. Two groups were then loaded to 25 MPa in unconfined compression. Half the specimens in each group were incubated in media supplemented with 8 mg/ml P 188 immediately after loading, while the other half was returned to standard media. At 1 and 24 It the percentages of live and dead cells in compressed and control groups were determined with a cell viability stain. At 1 It post-trauma, P188 incubated specimens had a significantly increased percentage of live cells in the superficial zone versus the no-P188 group. At 24 h the percentages of live cells in all three zones of the P188-treated explants were significantly greater than in the no treatment group. This study showed that P188 surfactant could help restore the integrity of cell membranes in cartilage damaged by blunt mechanical trauma. With the ability of P188 to "save" chondrocytes from early necrotic death using in vitro chondral explants, its role in prevention of a post-traumatic osteoarthritis in a diarthrodial joint should be further explored using in vivo animal models. (C) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.