Chemo-enzymatic synthesis of fluorinated 2-N-acetamidosugar nucleotides using UDP-GlcNAc pyrophosphorylase
Chemo-enzymatic synthesis of fluorinated 2-N-acetamidosugar nucleotides using UDP-GlcNAc pyrophosphorylase
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DOI：
10.1039/b402860k
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发表时间：
2004-01-01
影响因子：
3.2
通讯作者：
Nishimura, SI
中科院分区：
文献类型：
--
作者：
Feng, F;Okuyama, K;Nishimura, SI
两个非自然。用酶法合成了2-N-乙酰氨基糖核苷酸，即尿苷5 ′-二磷酸（UDP）2-乙酰氨基-2，4-二脱氧-4-氟-α-D-吡喃葡萄糖（UDP-4-FGlcNAc）1及其半乳糖异构体（UDP-4-FGalNAc）2。在尿苷5 ′-三磷酸（UTP）存在下，用UDP 2-乙酰氨基-2-脱氧-α-D-吡喃葡萄糖焦磷酸化酶将氟化2-N-乙酰氨基糖1-磷酸作为供体。
Two non-natural. uorinated 2-N-acetamidosugar nucleotides, uridine 5'-diphosphate (UDP) 2-acetamido-2,4-dideoxy-4-fluoro-alpha-D-glucopyranose (UDP-4-FGlcNAc) 1 and its galacto isomer (UDP-4-FGalNAc) 2, were enzymatically constructed by treating chemically synthesized. uorinated 2-N-acetamidosugar 1-phosphates as the donor with UDP 2-acetamido-2-deoxy-alpha-D-glucopyranose pyrophosphorylase in the presence of uridine 5'-triphosphate (UTP).