Virological features of hepatitis B virus-associated nephropathy in Japan
Virological features of hepatitis B virus-associated nephropathy in Japan
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DOI：
10.1002/jmv.20941
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发表时间：
2007-09-01
影响因子：
12.7
通讯作者：
Mizokami, Masashi
中科院分区：
文献类型：
--
作者：
Kusakabe, Atsunori;Tanaka, Yasuhito;Mizokami, Masashi
乙肝病毒相关性肾病被认为是一种免疫介导的疾病，依赖于病毒、宿主和环境因素之间的相互作用。但有关乙肝病毒相关性肾病发生发展与其基因分型及变异关系的研究报道较少。在日本，为了阐明肾病与HBV型之间的关系，我们检查了6名患有HBV型肾病的男性。直接测定HBV全基因组序列，并与国际数据库中检索到的共同序列[HBVA1(Aa)、A2(Ae)、B1(Bj)、B2(Ba)、C1(Cs)和C2(Ce)]进行比对，分析与乙肝相关肾病发生发展相关的特异性突变。6例患者的平均年龄为33.5岁。所有患者均检出HBeAg，且血清HBVdna水平较高。肾组织学检查提示膜性肾病5例，膜增生性肾小球肾炎1例。6例患者中，2例为HBVA1，2例为A2，2例为C2，提示以HBVA型为主。在2例HBVA1患者中观察到G1862T突变，导致前C区氨基酸由Valine(Val)转变为Phe(Phe)。只有一名患者有核心缺失。结论：HBV/A基因突变可能与膜性肾病有关，但其基因突变与乙肝相关性肾病的发生无明显关系。
Hepatitis B virus (HBV)-associated nephropathy is considered as an immune-mediated disorder which is dependent on interactions between viral, host, and environmental factors. But there are few reports that investigated the relationship between the development of HBV-associated nephropathy and HBV genotypes and the mutations. To clarify the relationship between nephropathy and HBV genotype in Japan, six male patients with HBV-associated nephropathy were examined. The complete genome sequences of HBV were determined directly and the specific mutations associated with the development of HBV-associated nephropathy were examined by comparison of the alignments along with consensus sequences [HBV/A1 (Aa), A2 (Ae), B1 (Bj), B2 (Ba), C1 (Cs) and C2 (Ce)] retrieved from international database. The mean age of the six patients was 33.5 years. HBeAg was found in all patients and serum HBV-DNA levels were relatively high. Histological findings of renal tissues indicated five cases of membranous nephropathy and one membranoproliferative glomerulonephritis. HBV genotypes from the six patients were two HBV/A1, two A2 and two C2, suggesting HBV/A was predominant. G1862T mutation was observed in the two HBV/A1 patients, resulting in the pre-core amino acid substitution with a switch from valine (Val) to phenylalanine (Phe). Only one patient had core deletions. It is concluded that HBV/A may be associated with membranous nephropathy, but little relationship between HBV gene mutations and the development of HBV-associated nephropathy was observed.