Axonal mRNA transport and localized translational regulation of κ-opioid receptor in primary neurons of dorsal root ganglia
Axonal mRNA transport and localized translational regulation of κ-opioid receptor in primary neurons of dorsal root ganglia
复制标题
DOI：
10.1073/pnas.0607394104
复制
发表时间：
2006-12-26
影响因子：
11.1
通讯作者：
Wei, Li-Na
中科院分区：
文献类型：
--
作者：
Bi, Jing;Tsai, Nien-Pei;Wei, Li-Na
κ-阿片受体（KOR）在突触前和突触后均可检测到，但KOR mRNA在感觉神经元突触前区室的亚细胞定位、翻译和调节仍然是未知的。原位杂交检测背根神经节（DRG）原代神经元kor mRNA的轴突分布。MS 2融合的GFP跟踪kor mRNA从DRG神经元索马体到轴突的运输，需要其5'和3' UTR。在Campenot室中，证实了DRG神经元的kor mRNA的轴突翻译，其依赖于其5' UTR，并被KCl去极化刺激。KCl去极化的DRG神经元呈现重新分配的kor mRNA从postpolysomal馏分的deletionally活性polysomal馏分。本研究为初级感觉神经元中非结构蛋白如KOR的mRNA转运和突触前蛋白合成的调节提供了证据，并证明了KCl去极化刺激轴突mRNA重新分布用于局部翻译调节的机制。
kappa-opioid receptor (KOR) is detected pre- and postsynaptically, but the subcellular localization, translation, and regulation of kor mRNA in presynaptic compartments of sensory neurons remain elusive. In situ hybridization detected axonal distribution of kor mRNA in primary neurons of dorsal root ganglia (DRG). The MS2-fused GFP tracked kor mRNA transport from DRG neuronal soma to axons, requiring its 5' and 3' UTRs. In Campenot chambers, axonal translation of kor mRNA was demonstrated for DRG neurons, which depended on its 5' UTR and was stimulated by KCl depolarization. KCl depolarization of DRG neurons rendered redistribution of kor mRNA from the postpolysomal fraction to the translationally active polysomal fraction. This study provided evidence for mRNA transport and regulation of presynaptic protein synthesis of nonstructural proteins like KOR in primary sensory neurons and demonstrated a mechanism of KCl depolarization-stimulated axonal mRNA redistribution for localized translational regulation.