Regulation of cellular behaviors of fibroblasts related to wound healing by sol-gel derived bioactive glass particles
Regulation of cellular behaviors of fibroblasts related to wound healing by sol-gel derived bioactive glass particles
复制标题
溶胶-凝胶衍生的生物活性玻璃颗粒对与伤口愈合相关的成纤维细胞的细胞行为的调节
DOI：
10.1002/jbm.a.35782
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发表时间：
2016
影响因子：
4.9
通讯作者：
Fu Xiaoling
中科院分区：
文献类型：
--
作者：
Xie Weihan;Chen Xiaofeng;Miao Guohou;Tang Jieying;Fu Xiaoling
溶胶-凝胶衍生的生物活性玻璃（BG）在皮肤修复的应用中具有巨大的潜力。然而，BG对皮肤细胞的具体调节仍不清楚，需要更多的研究。在此，我们合成了具有不同成分（60S、70S、80S 和 90S）的溶胶-凝胶衍生的 BG，发现 90S BG（90 mol% SiO2、6 mol% CaO、4 mol% P2O5）对正常人包皮成纤维细胞的增殖具有最佳支持作用。因此，以90S BG颗粒为模型，系统地研究成纤维细胞对BG的伤口愈合相关细胞反应。延时成像显示 90S BG 颗粒刺激了成纤维细胞运动。细胞外基质 (ECM) 相关基因的表达结果表明，90S BG 颗粒调节关键 ECM 分子的合成能力，包括 I 型胶原、III 型胶原、纤连蛋白和腱生蛋白-C。而且，90S BG颗粒极大地抑制了成纤维细胞的肌成纤维细胞分化。对细胞内信号通路的进一步分析表明，90S BG 颗粒通过 TGF-β1-Smad2 信号传导下调胶原合成和成纤维细胞向肌成纤维细胞的分化，TGF-β 受体 I 及其下游效应子 Smad2 的表达水平降低证明了这一点。我们的研究进一步了解了90S BG颗粒对成纤维细胞的特异性调节，这可能指导未来基于BG的伤口敷料的设计，并有利于BG颗粒在皮肤修复中的临床应用。 © 2016 Wiley periodicals, Inc. J Biomed Mater Res Part A: 104A: 2420–2429, 2016。
Sol–gel derived bioactive glass (BG) holds great potential in the application of skin repair. However, the specific regulation of BG on skin cells is still unclear and demands more investigation. Herein, we synthesized sol–gel derived BGs with different compositions (60S, 70S, 80S, and 90S) and found 90S BGs (90 mol % SiO2, 6 mol % CaO, 4 mol % P2O5) exhibited the best supportiveness for the proliferation of normal human foreskin fibroblasts. Thus, 90S BG particles were used as a model to systematically study the wound healing related cellular response of fibroblasts to BGs. Time‐lapse imaging revealed a promoted fibroblast motility stimulated by 90S BG particles. Results on the expression of extracellular matrix (ECM) related genes illustrated that 90S BG particles modulated the synthesis capacity for critical ECM molecules including type I collagen, type III collagen, fibronectin, and tenascin‐C. Moreover, the myofibroblastic differentiation of fibroblasts was greatly inhibited by 90S BG particles. Further analysis on the intracellular signaling pathways demonstrated that 90S BG particles down‐regulated the collagen synthesis and fibroblast‐to‐myofibroblast differentiation via TGF‐β1‐Smad2 signaling, evidenced by the decreased expression levels of TGF‐β receptor I and its downstream effector Smad2. Our study provided a further understanding of the specific regulation of 90S BG particles on fibroblasts, which may guide the future design of BG based wound dressing and benefit the clinical application of BG particles in skin repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2420–2429, 2016.