Inflammation modulates in vitro colonic myoelectric and contractile activity and interstitial cells of Cajal
Inflammation modulates in vitro colonic myoelectric and contractile activity and interstitial cells of Cajal
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DOI：
10.1152/ajpgi.1997.273.6.g1233
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发表时间：
1997-12-01
影响因子：
4.5
通讯作者：
Sarna, SK
中科院分区：
文献类型：
--
作者：
Lu, G;Qian, X;Sarna, SK
炎症抑制体内阶段性收缩活动。我们研究了炎症是否也会抑制体外阶段性收缩活动，如果是这样，这是否部分是由于Cajal间质细胞（ICC）的特定慢波特征和形态的改变。从正常和发炎的远端犬结肠获得环形肌条。通过粘膜暴露于乙醇和乙酸诱导炎症。自发的，乙酰甲胆碱诱导的，P物质诱导的，和电场刺激诱导的收缩的幅度较小，在发炎的肌条比在正常的肌条。炎症降低了环行肌细胞的静息膜电位和慢波的振幅和持续时间。炎症并不影响抑制性连接电位的幅度，但减少其持续时间。超微结构研究显示环形肌细胞之间的细胞外空间的扩大，ICC和相关神经结构的密度降低，ICC过程的损伤，其细胞质的空泡化和质膜的起泡。我们的结论是炎症引起的慢波特性的改变有助于抑制相位收缩。这些变化可能部分是由于对ICC的损害。炎症损害了肌源性和神经性的阶段性收缩调节。
Inflammation suppresses phasic contractile activity in vivo. We investigated whether inflammation also suppresses in vitro phasic contractile activity and, if so, whether this could in part be due to the alteration of specific slow wave characteristics and morphology of the interstitial cells of Cajal (ICC). Circular muscle strips were obtained from normal and inflamed distal canine colon. Inflammation was induced by mucosal exposure to ethanol and acetic acid. The amplitudes of spontaneous, methacholine-induced, substance P-induced, and electrical field stimulation-induced contractions were smaller in inflamed muscle strips than in normal muscle strips. Inflammation reduced the resting membrane potential and the amplitude and duration of slow waves in circular muscle cells. Inflammation did not affect the amplitude of inhibitory junction potentials but did decrease their duration. Ultrastructural studies showed expansion of the extracellular space between circular muscle cells, reduction in the density of ICC and associated neural structures, damage to ICC processes, vacuolization of their cytoplasm, and blebbings of the plasma membrane. We conclude that inflammation-induced alterations of slow wave characteristics contribute to the suppression of phasic contractions. These alterations may, in part, be due to the damage to ICC. Inflammation impairs both the myogenic and neural regulation of phasic contractions.