The inclusion of sub-detection limit LA-ICPMS data, in the analysis of otolith microchemistry, by use of a palindrome sequence analysis (PaSA)
The inclusion of sub-detection limit LA-ICPMS data, in the analysis of otolith microchemistry, by use of a palindrome sequence analysis (PaSA)
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DOI：
10.4319/lom.2007.5.97
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发表时间：
2007-03-01
影响因子：
2.7
通讯作者：
Kiflawi, M.
中科院分区：
文献类型：
--
作者：
Ben-Tzvi, O.;Abelson, A.;Kiflawi, M.
鱼的耳石是鱼类个体历史数据的可靠来源。从耳石中获得的光学和化学数据被用来揭示鱼的年龄、出生地区、扩散轨迹等。然而，尽管光学方法已经建立并广泛应用，获取和解释化学数据的方法仍在不断发展。尽管耳石微化学的分析方法在过去十年中取得了快速发展，但随着新的机会和限制的出现，耳石微化学的分析方法仍在继续发展。耳石化学通常反映环境条件，如环境水的化学成分、温度和盐度。然而，它们是如何相互作用产生耳石中观察到的化学特征的并不总是很清楚。这种知识上的差距和不一致部分可能是由于当测量的浓度低于习惯定义的检测限（DL）时，有价值的信息被丢弃。下面我们回顾一些在分析激光烧蚀电感耦合质谱（LA-ICPMS）获得的化学数据时可能出现的不准确性。我们认为，传统上定义为sub-DL的测量可以包含有价值的信息，否则这些信息将被丢弃。我们的论点基于从耳石岩心两侧获得的亚dl信号的分析。具体来说，我们表明这些信号通常形成与核心（回文）对称的序列，并且偶然获得最简单回文序列的概率远低于惯例的5%显著性水平。最后，我们讨论了如何以一种对浓度读数中的错误不敏感的方式分析这些微化学数据。
Fish otoliths are a reliable source of historical data regarding individual fish. Optical and chemical data obtained from otoliths are used to reveal the fish's age, natal areas, dispersal trajectories, and more. However, whereas optical methods are well established and widely practiced, methods for obtaining and interpreting chemical data continue to evolve. Despite rapid advances in the past decade, analytical approaches to otolith microchemisty continue to develop as new opportunities and limitations emerge. Otolith chemistry often reflects environmental conditions such as the chemical composition, temperature, and salinity of the ambient water. However, it is not always clear how these interact to produce the chemical signature observed in the otolith. Such gaps and inconsistencies in our knowledge may result partly from valuable information being discarded when measured concentrations fall below customarily defined detection limits (DL). Below we review some of the inaccuracies that may arise when analyzing chemical data obtained from laser-ablation inductively coupled mass spectrometry (LA-ICPMS). We argue that measurements traditionally defined as sub-DL can contain valuable information that would otherwise be discarded. We base our argument on the analysis of sub-DL signals obtained from both sides of otoliths core. Specifically, we show that these signals often form sequences that are symmetrical about the core ( palindrome), and that the probability of obtaining even the simplest palindromic sequence by chance falls well below the customary 5% significance level. We conclude by discussing how this microchemical data can be analyzed in a manner that is insensitive to errors in concentration readings.