Update and challenges on proteomics in rice
Update and challenges on proteomics in rice
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DOI：
10.1002/pmic.200600012
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发表时间：
2006-07
期刊：
影响因子：
3.4
通讯作者：
S. Komatsu;H. Yano
中科院分区：
文献类型：
--
作者：
S. Komatsu;H. Yano
水稻不仅是重要的农业资源，而且是生物学研究的模式植物。我们之前的综述强调了构建水稻蛋白质组数据库的不同方面，对不同组织和细胞器的水稻蛋白质进行编目，使用2-DE的差异蛋白质组学和鉴定的一些蛋白质的功能表征（Komatsu，S.，田中，N.，Proteomics 2005，5，938-949）。本文综述了蛋白质组学技术的优势和不足，以及目前水稻蛋白质组学研究中所使用技术的局限性。从这些技术中获得的关于蛋白质修饰、蛋白质-蛋白质相互作用和差异蛋白质组学新方法的发展的信息将有助于更精确地破译水稻中已知和/或未知蛋白质的功能。
Rice is not only an important agricultural resource but also a model plant for biological research. Our previous review highlighted different aspects of the construction of rice proteome database, cataloguing rice proteins of different tissues and organelle, differential proteomics using 2‐DE and functional characterization of some of the proteins identified (Komatsu, S., Tanaka, N., Proteomics 2005, 5, 938–949). In this review, the powerfulness and weaknesses of proteomic technologies as a whole and limitations of the currently used techniques in rice proteomics are discussed. The information obtained from these techniques regarding proteins modification, protein‐protein interaction and the development of new methods for differential proteomics will aid in deciphering more precisely the functions of known and/or unknown proteins in rice.