RNAi in mice: a promising approach to decipher gene functions in vivo
RNAi in mice: a promising approach to decipher gene functions in vivo
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DOI：
10.1016/j.biochi.2005.11.010
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发表时间：
2006-06-01
期刊：
影响因子：
3.9
通讯作者：
Deng, Chu-Xia
中科院分区：
文献类型：
--
作者：
Coumoul, Xavier;Deng, Chu-Xia
RNA干扰（RNAi）是一种简单而有效的研究基因功能的工具。已经开发出使用RNA聚合酶III启动子的基于载体的系统，以实现小干扰RNA（siRNA）或小发夹RNA（shRNA）在哺乳动物细胞中的稳定表达。最近的研究表明，当与Cre-loxP系统相结合时，基于载体的RNAi可以用于在小鼠中高效地实现内源基因的条件性或组织特异性敲低。在这里，我们回顾了这些最新进展，并讨论了这一新兴技术的优点，局限性和未来的发展。(c)2006年爱思唯尔SAS。All rights reserved.
RNA interference (RNAi) is a simple and powerful tool widely used to study gene functions in many species. Vector-based systems using RNA polymerase III promoters have been developed to achieve stable expression of small interfering RNA (siRNA) or small hairpin RNA (shRNA) in mammalian cells. Recent investigations demonstrated that when, combined with the Cre-loxP system, the vector-based RNAi can be used to achieve conditional or tissue specific knockdown of endogenous genes with high efficiency in mice. Here, we review these recent progresses and discuss the advantages, limitations and future development of this emerging technology. (c) 2006 Elsevier SAS. All rights reserved.