High-resolution mapping of a major and consensus quantitative trait locus for oil content to a ~ 0.8-Mb region on chromosome A08 in peanut (Arachis hypogaea L.)
High-resolution mapping of a major and consensus quantitative trait locus for oil content to a ~ 0.8-Mb region on chromosome A08 in peanut (Arachis hypogaea L.)
复制标题
花生 (Arachishypogaea L.) 染色体 A08 上 ~ 0.8-Mb 区域的含油量主要和共有数量性状位点的高分辨率图谱
DOI：
10.1007/s00122-019-03438-6
复制
发表时间：
2020-01-01
影响因子：
5.4
通讯作者：
Jiang, Huifang
中科院分区：
文献类型：
--
作者：
Liu, Nian;Guo, Jianbin;Jiang, Huifang
关键信息
基于ddRAD-seq的包含2595个位点的高密度遗传图谱鉴定了花生含油量的主效和共有QTL，该QTL在0.8-Mb物理区间内具有连锁标记。
摘要
提高花生含油量是花生育种的重要目标。利用连锁标记对数量性状基因座（QTL）进行高分辨率定位，有助于在育种中对目标性状进行标记辅助选择。以重组自交系（徐花13 ×中花6号）为材料，利用双酶切限制性内切酶位点相关DNA测序（ddRAD-seq）技术构建了小麦的遗传图谱。该图谱包含2595个位点，全长2465.62 cM，平均每个位点的遗传距离为0.95 cM。在5个连锁群上共检测到7个控制含油量的QTL，其中主效QTL qOCA08.1位于A08染色体上，解释的表型变异为10.14-27.19%。qOCA08.1的物理区间被进一步限定在约0.8Mb的基因组区域，其中两个影响油脂合成的基因已被注释。针对与含油量相关的SNP位点开发了标记SNPOCA 08，并在不同含油量的花生品种中进行了验证。在本研究中确定的主要和稳定的QTL可以进一步解剖基因发现。此外，该紧密连锁的含油量标记可用于花生分子标记辅助育种。
Key message
ddRAD-seq-based high-density genetic map comprising 2595 loci identified a major and consensus QTL with a linked marker in a 0.8-Mb physical interval for oil content in peanut.
Abstract
Enhancing oil content is an important breeding objective in peanut. High-resolution mapping of quantitative trait loci (QTLs) with linked markers could facilitate marker-assisted selection in breeding for target traits. In the present study, a recombined inbred line population (Xuhua 13 × Zhonghua 6) was used to construct a genetic map based on double-digest restriction-site-associated DNA sequencing (ddRAD-seq). The resulting high-density genetic map contained 2595 loci, and spanned a length of 2465.62 cM, with an average distance of 0.95 cM/locus. Seven QTLs for oil content were identified on five linkage groups, including the major and stable QTL qOCA08.1 on chromosome A08 with 10.14–27.19% phenotypic variation explained. The physical interval of qOCA08.1 was further delimited to a ~ 0.8-Mb genomic region where two genes affecting oil synthesis had been annotated. The marker SNPOCA08 was developed targeting the SNP loci associated with oil content and validated in peanut cultivars with diverse oil contents. The major and stable QTL identified in the present study could be further dissected for gene discovery. Furthermore, the tightly linked marker for oil content would be useful in marker-assisted breeding in peanut.