The cryptochrome (cry) gene and a mating isolation mechanism in tephritid fruit flies
The cryptochrome (cry) gene and a mating isolation mechanism in tephritid fruit flies
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DOI：
10.1534/genetics.104.028399
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发表时间：
2004-12-01
期刊：
影响因子：
3.3
通讯作者：
Raphael, KA
中科院分区：
文献类型：
--
作者：
An, X;Tebo, M;Raphael, KA
实蝇的两个兄弟种，Bactrocera，tryoni和Bactrocera neohumeralis，通过它们的交配时间来区分。这是由遗传学决定的，并且需要内源生物钟和光强度之间的相互作用。隐花色素（cry）基因，一个光敏感的组成部分的昼夜节律钟，分离在两个果实蝇物种。两个物种的推定氨基酸序列相同。在大脑中，原位杂交表明，哭是在中央大脑的PER免疫染色也观察到的侧部和背部区域，并在触角叶的外周细胞群中表达。在整个头、脑和触角中分析cry mRNA的水平。在整个头部，哭是丰富和不断表达。然而，在大脑和触角的转录周期丰富，在白天比晚上和哭转录的水平更高。在B的大脑和触角中更丰富。neohumeralis比B.引人注目的是，这些结果在由B之间的罕见交配产生的杂交系中重复。tryoni和B. neohumeralis，然后选择交配时间的基础上，这表明在交配隔离机制，区分物种的cry基因的作用。
Two sibling species of tephritid fruit fly, Bactrocera, tryoni and Bactrocera neohumeralis, are differentiated by their time of mating. which is genetical v determined and requires interactions between the endogenous, circadian clock and light intensity. The cryptochrome (cry) gene, a light-sensitive component of the circadian clock, was isolated in the two Bactrocera species. The putative amino acid sequence is identical in the two species. In the brain, in situ hybridization showed that cry is expressed in the lateral and dorsal regions of the central brain where PER immunostaining was also observed and in a peripheral cell cluster of the antennal lobes. Levels of cry mRNA were analyzed in whole head, brain, and antennae. In whole head, cry is abundantly and constantly expressed. However, in brain and antennae the transcript cycles in abundance, with higher levels during the day than at night and cry transcripts, are. more abundant in the brain and antennae of B. neohumeralis than in that of B. tryoni, Strikingly, these results are duplicated in hybrid lines, generated by rare mating between B. tryoni and B. neohumeralis and then selected on the basis of mating time, suggesting a role for the cry gene in the mating isolation mechanism that differentiates the species.