Soluble branched β-(1,4)Glucans from Acetobacter species show strong activities to induce interleukin-12 in vitro and inhibit T-helper 2 cellular response with immunoglobulin E production in vivo
Soluble branched β-(1,4)Glucans from Acetobacter species show strong activities to induce interleukin-12 in vitro and inhibit T-helper 2 cellular response with immunoglobulin E production in vivo
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DOI：
10.1074/jbc.m304948200
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发表时间：
2003-10-03
影响因子：
4.8
通讯作者：
Yoshikai, Y
中科院分区：
文献类型：
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作者：
Saito, K;Yajima, T;Yoshikai, Y
由产多糖醋杆菌（Acetobacter polysaccharogenes）产生的胞外多糖AC-1由具有葡糖基残基支链的β-（1，4）葡聚糖组成。我们发现，AC-1显示出强烈的活性，诱导产生白细胞介素-12 p40和肿瘤坏死因子-α的巨噬细胞系在体外。纤维素酶处理完全消除AC-1诱导巨噬细胞产生肿瘤坏死因子-α的活性，而用多粘菌素B或蛋白酶处理AC-1不影响活性。使用Toll样受体（TLR）4缺陷小鼠和TLR 4转染的人细胞系的实验结果表明，TLR 4参与AC-1的模式识别。在用OVA免疫的小鼠中，AC-1的体内给药显著降低了卵清蛋白（OVA）特异性IgE的血清水平和响应于OVA的T细胞产生的白细胞介素-4。AC-1是一种可溶性支链β-（1，4）葡聚糖，可用于预防和治疗伴随IgE产生的过敏性疾病。
An extracellular polysaccharide, AC-1, produced by Acetobacter polysaccharogenes is composed of beta-(1,4) glucan with branches of glucosyl residues. We found that AC-1 showed a strong activity to induce production of interleukin-12 p40 and tumor necrosis factor-alpha by macrophage cell lines in vitro. Cellulase treatment completely abolished the activity of AC-1 to induce tumor necrosis factor-alpha production by macrophages, whereas treatment of AC-1 with polymyxin B or proteinase did not affect the activity. Results of experiments using Toll-like receptor (TLR) 4-deficient mice and TLR4-transfected human cell line indicated that TLR4 is involved in pattern recognition of AC-1. In vivo administration of AC-1 significantly reduced the serum levels of ovalbumin (OVA)-specific IgE and interleukin- 4 production by T cells in response to OVA in mice immunized with OVA. AC-1, a soluble branched beta-(1,4) glucan may be useful in prevention and treatment of allergic disorders with IgE production.