Isolation and characterization of a chimpanzee alphaherpesvirus
Isolation and characterization of a chimpanzee alphaherpesvirus
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DOI：
10.1099/vir.0.81606-0
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发表时间：
2006-01-01
影响因子：
3.8
通讯作者：
Eberle, R
中科院分区：
文献类型：
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作者：
Luebcke, E;Dubovi, E;Eberle, R
虽然已分离出类人猿特有的β和γ疱疹病毒，但迄今为止，从类人猿中分离出的所有α疱疹病毒已被证明是人类病毒[单纯疱疹病毒1型（HSV1）和2型（HSV2）或水痘带状疱疹病毒]。如果阿尔法疱疹病毒与它们的宿主物种共同进化，那么某些（如果不是所有）类人猿物种应该携带它们自己的阿尔法疱疹病毒。在这里，从黑猩猩（ChHV）的分离和特征描述。ChHV基因组中许多基因的测序表明它与HSV共线。系统发育分析将ChHV与HSV1和HSV2放在一个分支中，后者是旧大陆猴子的字母疱疹病毒，构成一个单独的分支。竞争ELISA法对人类hsv2免疫血清和黑猩猩chhv免疫血清的反应模式进行了分析，证实了这一关系。系统发育分析也将ChHV而不是HSV1作为HSV2的近亲。
Although both beta- and gammaherpesviruses indigenous to great-ape species have been isolated, to date all alphaherpesviruses isolated from apes have proven to be human viruses [herpes simplex virus types 1 (HSV1) and 2 (HSV2) or varicella-zoster virus]. If the alphaherpesviruses have co-evolved with their host species, some if not all ape species should harbour their own alphaherpesviruses. Here, the isolation and characterization of an alphaherpesvirus from a chimpanzee (ChHV) are described. Sequencing of a number of genes throughout the ChHV genome indicates that it is collinear with that of HSV. Phylogenetic analyses place ChHV in a clade with HSV1 and HSV2, the alphaherpesviruses of Old World monkeys comprising a separate clade. Analysis of reactivity patterns of HSV2-immune human sera and ChHV-immune chimpanzee sera by competition ELISA support this relationship. Phylogenetic analyses also place ChHV rather than HSV1 as the closest relative of HSV2.