Diagnosis of bacterial endophthalmitis by broad-range quantitative PCR
Diagnosis of bacterial endophthalmitis by broad-range quantitative PCR
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DOI：
10.1136/bjo.2009.171504
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发表时间：
2011-03-01
影响因子：
4.1
通讯作者：
Mochizuki, Manabu
中科院分区：
文献类型：
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作者：
Sugita, Sunao;Shimizu, Norio;Mochizuki, Manabu
目的检测19例可疑细菌性眼内炎患者眼内液中细菌基因组，分析其与感染性眼内炎的临床相关性。还收集了来自葡萄膜炎患者的50个眼部样品，沿着来自没有眼部炎症的患者的40个样品并用作对照。结果19例临床可疑细菌性眼内炎患者中18例（95%）检出细菌16 SrDNA。除1例患者外，在这些患者中检测到高拷贝数的细菌DNA（1.7x10（3）-1.7x10（9）拷贝/ml）。有10个样本（53%）细菌培养阳性，而有9个样本（47%）革兰氏染色阳性。实时PCR检测到细菌16 S rDNA在三个（6%）的50个样本，从控制葡萄膜炎患者。结论细菌16 SrDNA定量PCR是诊断细菌性眼内炎的有效方法。
Aim To measure the bacterial genome in ocular fluids and to analyse the clinical relevance of infectious endophthalmitis.Methods Nineteen ocular fluid samples (eight aqueous humour and 11 vitreous fluid samples) were collected from 19 patients with suspected bacterial endophthalmitis. Fifty ocular samples from uveitis patients were also collected along with 40 samples from patients without ocular inflammation and used as controls. Bacterial ribosomal DNA (16S rDNA) was measured by a quantitative PCR assay.Results Bacterial 16S rDNA was detected in patients with clinically suspected bacterial endophthalmitis (18/19, 95%). With the exception of one case, high copy numbers of bacterial DNA were detected (1.7x10(3)-1.7x10(9) copies/ml) in these patients. There were 10 samples (53%) with positive bacterial cultures while there were nine samples (47%) with positive Gram-staining. Real-time PCR detected bacterial 16S rDNA in three (6%) of the 50 samples from the control uveitis patients. In addition, none of the samples from the control patients without intraocular inflammation were positive.Conclusions Quantitative broad-range PCR of bacterial 16S rDNA is a useful tool for diagnosing bacterial endophthalmitis.