Transport of sphingomyelin to the cell surface is inhibited by brefeldin A and in mitosis, where C6-NBD-sphingomyelin is translocated across the plasma membrane by a multidrug transporter activity.
Transport of sphingomyelin to the cell surface is inhibited by brefeldin A and in mitosis, where C6-NBD-sphingomyelin is translocated across the plasma membrane by a multidrug transporter activity.
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鞘磷脂向细胞表面的转运受到布雷菲德菌素 A 和有丝分裂的抑制，其中 C6-NBD-鞘磷脂通过多药物转运蛋白活性跨质膜转运。
作者：
A. van Helvoort;M. Giudici;M. Thielemans;G. van Meer
鞘磷脂是哺乳动物细胞表面的主要脂质。鞘磷脂在高尔基腔内合成后到达载体囊泡内部的质膜外小叶的观点受到运输研究结果不一致的挑战。为了研究鞘磷脂是否存在通往细胞表面的替代途径，使用brefeldin A和有丝分裂细胞阻断高尔基复合体和质膜之间的囊泡交通。外源性鞘磷脂酶应用于低温下测定CHO细胞表面鞘磷脂的存在。在37℃下，新合成的放射性标记鞘磷脂在1.5小时内与细胞表面鞘磷脂达到平衡。Brefeldin A和有丝分裂抑制了这种运输，但令人惊讶的是，短链鞘磷脂类似物N-6[7-硝基-2,1,3-苯并二唑-4-基]氨基己醇(C6-NBD)-鞘磷脂的表面形态并没有受到影响，这是通过清除白蛋白在培养基中消耗这种脂质来检测的。多药耐药p -糖蛋白抑制剂环孢素A和PSC 833阻断了brefeldin A存在下c6 - nbd -鞘磷脂的转运。在HepG2和HeLa细胞以及短链葡萄糖神经酰胺中也观察到同样的情况，这表明了转运蛋白依赖性鞘脂跨质膜转运的一般性质。
Sphingomyelin is a major lipid of the mammalian cell surface. The view that sphingomyelin, after synthesis in the Golgi lumen, reaches the outer leaflet of the plasma membrane on the inside of carrier vesicles has been challenged by inconsistencies in the results of transport studies. To investigate whether an alternative pathway to the cell surface exists for sphingomyelin, brefeldin A and mitotic cells were used to block vesicular traffic between the Golgi complex and the plasma membrane. Exogenous sphingomyelinase was applied in the cold to assay for the presence of sphingomyelin on the surface of CHO cells. Newly synthesized radiolabeled sphingomyelin was found to equilibrate with cell surface sphingomyelin within 1.5 hours at 37 degrees C. Brefeldin A and mitosis inhibited this transport but, surprisingly, not the surface appearance of the short-chain sphingomyelin analog N-6[7-nitro-2,1,3-benzoxadiazol-4-yl]aminohexanoyl(C6-NBD)-sphingo myelin as assayed by depletion of this lipid in the medium by the scavenger albumin. Transport of C6-NBD-sphingomyelin in the presence of brefeldin A was blocked by cyclosporin A and PSC 833, inhibitors of the multidrug resistance P-glycoprotein. The same was observed in HepG2 and HeLa cells, and for short-chain glucosylceramide, which demonstrates the general nature of the transporter-dependent sphingolipid translocation across the plasma membrane.
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DOI：
10.1016/s0021-9258(19)38937-9
发表时间：
1990-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Futerman;B. Stieger;B. Stieger;A. Hubbard;R. Pagano
通讯作者：
A. Futerman;B. Stieger;B. Stieger;A. Hubbard;R. Pagano
在上皮细胞（Madin-Darby 犬肾）基底外侧表面上，二酰甘油转化为磷脂酰胆碱。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
vanHelvoort,A;van'tHof,W;Ritsema,T;Sandra,A;vanMeer,G
通讯作者：
vanMeer,G
DOI：
10.1016/s0021-9258(17)31548-x
发表时间：
1994-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Linardic;Y. Hannun
通讯作者：
C. Linardic;Y. Hannun
胆固醇和水泡性口炎病毒G蛋白从内质网到质膜采取不同的途径。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Urbani,L;Simoni,RD
通讯作者：
Simoni,RD
有丝分裂期间的脂质运输。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kobayashi,T;Pagano,RE
通讯作者：
Pagano,RE