Affinity Labeling and purification of plant chitin-binding LysM-receptor with chitin octasaccharide derivatives, Lectin Purification and Analysis, Springer Nature, edited by Jun Hirabayashi
Affinity Labeling and purification of plant chitin-binding LysM-receptor with chitin octasaccharide derivatives, Lectin Purification and Analysis, Springer Nature, edited by Jun Hirabayashi
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植物几丁质结合 LysM 受体与几丁质八糖衍生物的亲和标记和纯化，凝集素纯化和分析，Springer Nature，Jun Hirabayashi 编辑
DOI：
10.1007/978-1-0716-0430-4_39
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Kaku H.
中科院分区：
文献类型：
--
作者：
Shinya T.;Shibuya N.;Kaku H.
赖氨酸基序（LysM）是存在于所有界域中的碳水化合物结合模块。LysM与含N-乙酰葡糖胺的分子如肽聚糖、几丁质、Nod因子和Myc因子结合，并存在于肽聚糖水解酶、几丁质酶和植物病原体效应物以及植物受体/共受体中，用于防御和共生信号传导。本章介绍了非放射性几丁质配体的合成，生物素化几丁质八糖，（GlcNAc）8-Bio，及其应用的检测和表征几丁质结合LysM受体CEBiP在微粒体膜部分水稻悬浮培养细胞的亲和标记。我们还描述了用合成的（GlcNAc）8-APEA-CH-Sepharose作为亲和基质，通过亲和层析从水稻细胞质膜中纯化CEBiP。
Lysin motif (LysM) is a carbohydrate-binding modules found in all kingdoms. LysM binds toN-acetylglucosamine-containing molecules such as peptidoglycan, chitin, Nod factor, and Myc factor and is found in peptidoglycan hydrolases, chitinases, and plant pathogen effectors and plant receptor/co-receptor for defense and symbiosis signaling. This chapter describes the synthesis of a nonradioactive chitin ligand, biotinylated chitin octasaccharide, (GlcNAc)8-Bio, and its application for the detection and characterization of chitin-binding LysM receptor CEBiP in the microsomal membrane fraction of rice suspension-cultured cells by affinity labeling. We also describe the purification of CEBiP from the plasma membrane of the rice cells by affinity chromatography with the synthesized (GlcNAc)8-APEA-CH-Sepharose as an affinity matrix.