Methods and application of coherent X-ray diffraction imaging of noncrystalline particles
Methods and application of coherent X-ray diffraction imaging of noncrystalline particles
复制标题
DOI：
10.1007/s12551-020-00690-9
复制
发表时间：
2020-03
影响因子：
--
通讯作者：
M. Nakasako;Amane Kobayashi;Y. Takayama;K. Asakura;Mao Oide;K. Okajima;Tomotaka Oroguchi;Masaki Yamamoto
中科院分区：
文献类型：
--
作者：
M. Nakasako;Amane Kobayashi;Y. Takayama;K. Asakura;Mao Oide;K. Okajima;Tomotaka Oroguchi;Masaki Yamamoto
显微成像技术已被开发用于可视化生物细胞中发生的事件。相干X射线衍射成像是适用于从未结晶的细胞和细胞器结构分析的技术之一。在实验中，单个非晶粒子被几乎完全空间相干性的 X 射线束照射。原则上，沿着光束方向投射的粒子的结构是通过使用迭代相位检索算法单独从精细记录的衍射图案中检索的。在这里，我们描述了相干X射线衍射成像的基本理论和实验方法，以及利用日本8-Gev超级光子环和SPring-8安斯特罗姆紧凑自由电子激光器提供的X射线在非晶样品结构研究中的最新应用。
Microscopic imaging techniques have been developed to visualize events occurring in biological cells. Coherent X-ray diffraction imaging is one of the techniques applicable to structural analyses of cells and organelles, which have never been crystallized. In the experiment, a single noncrystalline particle is illuminated by an X-ray beam with almost complete spatial coherence. The structure of the particle projected along the direction of the beam is, in principle, retrieved from a finely recorded diffraction pattern alone by using iterative phase-retrieval algorithms. Here, we describe fundamental theory and experimental methods of coherent X-ray diffraction imaging and the recent application in structural studies of noncrystalline specimens by using X-rays available at Super Photon Ring of 8-Gev and SPring-8 Angstrom Compact Free Electron Laser in Japan.