APIGENIN AND TANGERETIN ENHANCE GAP JUNCTIONAL INTERCELLULAR COMMUNICATION IN RAT-LIVER EPITHELIAL-CELLS
APIGENIN AND TANGERETIN ENHANCE GAP JUNCTIONAL INTERCELLULAR COMMUNICATION IN RAT-LIVER EPITHELIAL-CELLS
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DOI：
10.1093/carcin/15.10.2325
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发表时间：
1994-10-01
期刊：
影响因子：
4.7
通讯作者：
MARTEL, P
中科院分区：
文献类型：
--
作者：
CHAUMONTET, C;BEX, V;MARTEL, P
研究了两种黄酮（芹菜素和橘子素）在大鼠肝上皮细胞系 REL 中调节间隙连接细胞间通讯 (GJIC) 的能力。它们的细胞毒性首先通过细胞密度和中性红摄取测定来确定：芹菜素和橘皮素在我们实验中使用的浓度10和25μM时都没有细胞毒性。然后，我们使用染料转移测定研究了 GJIC，我们观察到芹菜素和橘子素都增强了它，在 25 μM 24 小时内达到最大刺激 (x 1.7-1.8)。当染料转移增强时，连接蛋白 43 的量增加，这一点通过蛋白质印迹和免疫荧光分析得到证明。仅对于芹菜素，Northern 印迹分析显示连接蛋白 43 mRNA 的积累。此外，将 REL 细胞与两种化合物一起孵育 1 或 24 小时，可防止 12-O-十四烷酰佛波醇-13-乙酸酯（1 或 10 ng/ml）对染料转移的抑制。芹菜素增强GJIC可能是芹菜素体内抗肿瘤促进作用的主要机制之一。至于橘皮素，其增强 GJIC 的能力完善了其对引发后过程的潜在保护特性。
Two flavones, apigenin and tangeretin, were studied for their ability to modulate gap junctional intercellular communication (GJIC) in the rat liver epithelial cell line REL. Their cytotoxicity was first determined by cell density and neutral red uptake assays: neither apigenin nor tangeretin are cytotoxic at 10 and 25 mu M, the concentrations used in our experiments. We then studied GJIC using the dye transfer assay and we observed that both apigenin and tangeretin enhance it, the maximum stimulation (x 1.7-1.8) being achieved at 25 mu M for 24 h. When the dye transfer was enhanced, the amount of connexin 43 increased, which was demonstrated by Western blot and immunofluorescence analysis. For apigenin only, Northern blot analysis showed an accumulation of connexin 43 mRNA. In addition, the incubation of REL cells with the two compounds, for 1 or 24 h, prevented the inhibition of dye transfer by 12-O-tetradecanoylphorbol-13-acetate (1 or 10 ng/ml). The enhancement of GJIC by apigenin could be one of the major mechanisms responsible for apigenin's anti-tumour promoting action in vivo. As for tangeretin, its capacity to enhance GJIC completes its potential protective properties towards the post-initiation process.