A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress
A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress
复制标题
芜菁中BrrWSD1的剪接变体及其在干旱胁迫下蜡酯合成中的可能作用
DOI：
10.1021/acs.jafc.9b04069
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发表时间：
2019
影响因子：
6.1
通讯作者：
Yang Yongping
中科院分区：
文献类型：
--
作者：
Yang Yunqiang;Pu Yanan;Yin Xin;Du Jiancan;Zhou Zhili;Yang Danni;Sun Xudong;Sun Hang;Yang Yongping
Cuticular wax accumulation in plants contributes to drought tolerance. Here, we compared the drought levels on two varieties with different genotypes in turnip (Brassica rapavar. rapa) and found that the drought tolerance was higher in the waxy KTRG-B48a than in the wax-free KTRG-B48b. A combination of PacBio and Illumina sequencing analyses revealed that differential transcripts were mainly enriched in the wax synthesis pathway, and a splice variant (BrrWSD1-X2) was identified in the waxy KTRG-B48a.BrrWSD1-X2had a stronger ability to synthesize wax esters thanBrrWSD1-X1using heterologous expression in yeast (Saccharomyces cerevisiae) mutant H1246a. Then, we speculated that the T to C transversion of the third intron and the higher number of TA repeats in the third intron ofBrrWSD1DNA in the waxy KTRG-B48a may result in a lower efficiency of splicing recognition of the third intron, resulting in the emergence ofBrrWSD1-X2in waxy varieties.
