Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study.
Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study.
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DOI：
10.1155/2021/7967552
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发表时间：
2021
影响因子：
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通讯作者：
Aouizerat B
中科院分区：
文献类型：
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作者：
Gonzalez-Mercado VJ;Lim J;Saligan LN;Perez N;Rodriguez C;Bernabe R;Ozorio S;Pedro E;Sepehri F;Aouizerat B
肠道微生物区系组成的改变(称为生物失调)与抑郁症状的病理生物学有关；然而，证据仍然有限。这项横断面先导性研究旨在探讨直肠癌新辅助化疗和放射治疗期间抑郁症状评分是否发生变化，以及肠道微生物类群丰度和预测的功能通路是否与化疗和放射治疗结束时的抑郁症状相关。采用汉密尔顿抑郁量表(HAM-D)对40例新诊断的直肠癌患者(年龄28~81岁，男性23例)进行抑郁症状评估，并提供粪便样本进行16S rRNA测序。使用QIIME2分析肠道微生物组数据，并对R组进行相关性和回归分析。与CRT前相比，参与者在治疗结束时抑郁症状显著增加。金黄色葡萄球菌、芽孢杆菌XI科、放线菌、链球菌、乳球菌、卫塞拉和明串珠菌科的相对丰度与HAM-D评分呈显著正相关(Spearman‘s Rho=0.42~0.32)，而链球菌、乳杆菌、链球菌、瘤胃杆菌、瘤胃球菌、副杆菌的相对丰度与HAM-D评分呈负相关(Spearman’s Rho=−0.43 ~−0.31)。在CRT结束时与抑郁症状评分相关的14个预测MetaCyc通路中，11个(79%)与生物合成通路有关。重要的细菌分类和预测的功能通路与直肠癌化疗和放射治疗结束时的抑郁症状相关，这值得进一步检查和复制我们的发现。
The role of alterations in gut microbiota composition (termed dysbiosis) has been implicated in the pathobiology of depressive symptoms; however, evidence remains limited. This cross-sectional pilot study is aimed at exploring whether depressive symptom scores changed during neoadjuvant chemotherapy and radiation therapy to treat rectal cancer, and if gut microbial taxa abundances and predicted functional pathways correlate with depressive symptoms at the end of chemotherapy and radiation therapy. 40 newly diagnosed rectal cancer patients (ages 28-81; 23 males) were assessed for depressive symptoms using the Hamilton Rating Scale for Depression (HAM-D) and provided stool samples for 16S rRNA sequencing. Gut microbiome data were analyzed using QIIME2, and correlations and regression analyses were performed in R. Participants had significantly higher depressive symptoms at the end as compared to before CRT. The relative abundances of Gemella, Bacillales Family XI, Actinomyces, Streptococcus, Lactococcus, Weissella, and Leuconostocaceae were positively correlated (Spearman's rho = 0.42 to 0.32), while Coprobacter, Intestinibacter, Intestimonas, Lachnospiraceae, Phascolarctobacterium, Ruminiclostridium, Ruminococcaceae (UCG-005 and uncultured), Tyzzerella, and Parasutterella (Spearman's rho = −0.43 to − 0.31) were negatively correlated with HAM-D scores. Of the 14 predicted MetaCyc pathways that correlated with depressive symptom scores at the end of CRT, 11 (79%) were associated with biosynthetic pathways. Significant bacterial taxa and predicted functional pathways correlated with depressive symptoms at the end of chemotherapy and radiation therapy for rectal cancer which warrants further examination and replication of our findings.